Optimization of explants, media, plant growth regulators and carbohydrates on callus induction and plant regeneration in Citrus jambhiri Lush.

被引:0
作者
Sidhu, G. S. [1 ]
Malhotra, P. K. [1 ]
Rattanpal, H. S. [2 ]
机构
[1] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana 141004, Punjab, India
[2] Punjab Agr Univ, Dept Fruit Sci, Ludhiana 141004, Punjab, India
来源
JOURNAL OF HORTICULTURAL SCIENCES | 2023年 / 18卷 / 01期
关键词
SOMATIC EMBRYOGENESIS; CULTURE; DIFFERENTIATION; TISSUE; STIMULATION; CELLS;
D O I
10.24154/jhs.v18i1.2159
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Callus induction was attempted from the four explants viz. root, cotyledon, epicotyl, and leaf segments excised from in vitro raised seedlings of C. jambhiri. Among various MS media supplementations with growth regulators and carbohydrates, the maximum (95.50%) and the earliest (8.30 days) callogenesis was obtained in epicotyl segments, when cultured on MS medium supplemented with NAA (10.0 mgl(-1)) + BAP (1.0 mgl(-1)) + sucrose (8%). The modified MS (macro and micro-nutrients reduced to half) fortified with BAP (5.0 mgl(-1)) + GA(3) (3.0 mgl(-1)) recorded maximum shoot regeneration (43.10%) from callus, with an average of 5.30 shoots per callus after 35.50 days of culturing. However, prolonged exposure to GA(3) resulted in thin elongated shoots and leaves. The age of the callus substantially influenced the plant regeneration frequency. The potency of the callus to regenerate decreased significantly with an increase in the age of the callus. Shoot regeneration was recorded maximum (43.43%) in 60 days old calli, followed by 90 days old (30.48%) calli, whereas it was minimum (10.46%) in 150 days old calli. The maximum (79.50%) shoot proliferation was recorded in MS medium supplemented with BAP (1.0 mgl(-1)) + Kin (0.5 mgl(-1)) with an average of 5.06 shoots per culture. The MS medium fortified with NAA (1.0 mgl(-1)) + IBA (1.0 mgl(-1)) induced maximum (77.33%) rooting, with an average of 3.19 roots per shoot after 13.4 days of culturing. Rooted plants were hardened and survived the best (83.6%) on the potting mixture consisting of cocopeat + vermiculite + perlite (2:1:1).
引用
收藏
页码:162 / 172
页数:11
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