Whole Animal Feeding FLat (WAFFL): a complete and comprehensive validation of a novel, high-throughput fly experimentation system

被引：2

作者：

Jaime, Maria D. L. A. ^{[1
,2
]}

Salem, Ghadi H. ^{[3
]}

Martinez, Daniel J. ^{[1
]}

Karott, Sean ^{[2
]}

Flores, Alejandra ^{[2
,4
]}

Palmer, Cameron D. ^{[2
]}

Mahadevaraju, Sharvani ^{[2
,5
]}

Krynitsky, Jonathan ^{[3
]}

Garmendia-Cedillos, Marcial ^{[3
]}

Anderson, Sarah ^{[3
]}

Harbison, Susan ^{[6
]}

Pohida, Thomas J. ^{[3
]}

Ludington, William B. ^{[1
,7
]}

Oliver, Brian ^{[2
,5
]}

机构：

[1] Carnegie Inst Washington, Dept Embryol, Baltimore, MD 21218 USA

[2] NIDDKD, Lab Cellular & Dev Biol, NIH, 50 South Dr, Bethesda, MD 20814 USA

[3] NIBIB, Instrument Dev & Engn Applicat Solut, NIH, 12 South Dr, Bethesda, MD 20892 USA

[4] Case Western Reserve Univ, Dept Physiol & Biophys, 10900 Euclid Ave, Cleveland, OH 44106 USA

[5] NIDDKD, Lab Biochem & Genet, NIH, 50 South Dr, Bethesda, MD 20814 USA

[6] NHLBI, Lab Syst Genet, NIH, 10 Ctr Dr, Bethesda, MD 20814 USA

[7] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

G3-GENES GENOMES GENETICS | 2023年 / 13卷 / 03期

关键词：

Drosophila; high-throughput; feeding behavior; screening; DRUG DISCOVERY; DROSOPHILA; RESOURCES; TRACKING; PLATFORM; GENE; FOOD;

D O I：

10.1093/g3journal/jkad012

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Non-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melanogaster in screening, we designed the Whole Animal Feeding FLat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high-throughput format. Our 3D printed system is compatible with inexpensive and readily available, commercial 96-well plate consumables and equipment. Experimenters can change the diet at will during the experiment and video record for behavior analysis, enabling precise dosing, measurement of feeding, and analysis of behavior in a 96-well plate format.

引用

页数：12

共 37 条

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