Immotile cilia mechanically sense the direction of fluid flow for left-right determination

被引：65

作者：

Katoh, Takanobu A. ^{[1
,2
]}

Omori, Toshihiro ^{[3
]}

Mizuno, Katsutoshi ^{[1
,8
]}

Sai, Xiaorei ^{[1
]}

Minegishi, Katsura ^{[1
,9
]}

Ikawa, Yayoi ^{[1
]}

Nishimura, Hiromi ^{[1
]}

Itabashi, Takeshi ^{[4
]}

Kajikawa, Eriko

Hiver, Sylvain

Iwane, Atsuko H.

Ishikawa, Takuji ^{[3
]}

Okada, Yasushi ^{[5
,6
,7
]}

Nishizaka, Takayuki

Hamada, Hiroshi ^{[1
]}

机构：

[1] RIKEN Ctr Biosyst Dynam Res, Lab Organismal Patterning, Kobe, Hyogo, Japan

[2] Gakushuin Univ, Fac Sci, Dept Phys, Toshima ku, Tokyo, Japan

[3] Tohoku Univ, Grad Sch Biomed Engn, Aoba Aramaki ku, Sendai, Miyagi, Japan

[4] RIKEN Ctr Biosyst Dynam Res, Higashihiroshima, Hiroshima, Japan

[5] RIKEN Ctr Biosyst Dynam Res, Lab Cell Polar Regulat, Suita, Osaka, Japan

[6] Univ Tokyo, Universal Biol Inst, Dept Cell Biol & Phys, Tokyo, Japan

[7] Univ Tokyo, Int Res Ctr Neurointelligence, Tokyo, Japan

[8] Univ Fukui, Fac Med Sci, Dept Cell Biol & Biochem, Div Med, Eiheiji cho, Fukui, Japan

[9] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Mol Therapy, Tokyo, Japan

来源：

SCIENCE | 2023年 / 379卷 / 6627期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

LEFT-RIGHT ASYMMETRY; LEFT-RIGHT AXIS; NODE; MOUSE;

D O I：

10.1126/science.abq8148

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Immotile cilia at the ventral node of mouse embryos are required for sensing leftward fluid flow that breaks left-right symmetry of the body. However, the flow-sensing mechanism has long remained elusive. In this work, we show that immotile cilia at the node undergo asymmetric deformation along the dorsoventral axis in response to the flow. Application of mechanical stimuli to immotile cilia by optical tweezers induced calcium ion transients and degradation of Dand5 messenger RNA (mRNA) in the targeted cells. The Pkd2 channel protein was preferentially localized to the dorsal side of immotile cilia, and calcium ion transients were preferentially induced by mechanical stimuli directed toward the ventral side. Our results uncover the biophysical mechanism by which immotile cilia at the node sense the direction of fluid flow.

引用

页码：66 / +

页数：6

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