Armillaria ostoyae extracts inhibit EMT of cancer cell lines via TGF-β and Wnt/β-catenin signaling components

被引：2

作者：

Jovanovic, Milena ^{[1
,2
,4
]}

Virijevic, Katarina ^{[2
]}

Grujovic, Mirjana ^{[2
]}

Ciric, Andrija ^{[1
]}

Petrovic, Ivica ^{[3
]}

Arsenijevic, Dejan ^{[1
]}

Zivanovic, Marko ^{[2
]}

Ljujic, Biljana ^{[3
]}

Seklic, Dragana ^{[2
]}

机构：

[1] Univ Kragujevac, Fac Sci, Radoja Domanovica 12, Kragujevac 34000, Serbia

[2] Univ Kragujevac, Inst Informat Technol Kragujevac, Jovana Cvijica Bb, Kragujevac 34000, Serbia

[3] Univ Kragujevac, Fac Med Sci, Radoja Domanovica 69, Kragujevac 34000, Serbia

[4] Univ Kragujevac, Fac Sci, Dept Biol & Ecol, Radoja Domanovica 12, Kragujevac 34000, Serbia

来源：

FOOD BIOSCIENCE | 2024年 / 57卷

关键词：

Fungotherapy; Migration; Phenolics; Sinapic acid; Smad2; EPITHELIAL-MESENCHYMAL TRANSITION; COLORECTAL-CANCER;

D O I：

10.1016/j.fbio.2023.103250

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Armillaria ostoyae is an edible mushroom, appreciated in human nutrition, with several medicinal properties already proven. However, its phenolic composition and effects on cancer have never been investigated so far, especially regarding specific signaling pathways included in first steps of cancer metastatic process, epithelialmesenchymal transition (EMT) and migration. We report phenolic profile of two Armillaria ostoyae extracts, ethanol and diethyl ether (E and DE) and their effects on colorectal carcinoma (HCT-116) and cervical adenocarcinoma (HeLa) cells. Phenolics were detected using HPLC, while effects on cancer cells were investigated using MTT test; expression of markers related to EMT on gene and protein level by flow cytometry, immunofluorescence and qRT-PCR method. Antimigratory potential was assessed on collective and individual type of migration by Wound healing assay and RTCA technique. Sinapic acid was detected as the main phenolic component in E, while rutin and naringin were dominant in DE type of extract. Extracts notably suppressed proEMT markers: vimentin, N-cadherin, beta-catenin, Snail, Smad2 and MMP-9, and elevated anti-EMT marker Ecadherin. Consequently, collective and single cell migration were significantly inhibited via regulation of TGF-beta and Wnt/beta-catenin signaling components. DE extract was more efficient in suppression of EMT and migration of colorectal and cervical cancer cells.

引用

页数：10

共 33 条

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