Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data-Independent Acquisition

被引:71
作者
Heil, Lilian R. [1 ]
Damoc, Eugen [2 ]
Arrey, Tabiwang N. [2 ]
Pashkova, Anna [2 ]
Denisov, Eduard [2 ]
Petzoldt, Johannes [2 ]
Peterson, Amelia C. [2 ]
Hsu, Chris [1 ]
Searle, Brian C. [3 ,4 ]
Shulman, Nicholas [1 ]
Riffle, Michael [1 ]
Connolly, Brian [1 ]
Maclean, Brendan X. [1 ]
Remes, Philip M. [5 ]
Senko, Michael W. [5 ]
Stewart, Hamish I. [2 ]
Hock, Christian [2 ]
Makarov, Alexander A. [2 ]
Hermanson, Daniel [5 ]
Zabrouskov, Vlad [5 ]
Wu, Christine C. [1 ]
Maccoss, Michael J. [1 ]
机构
[1] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA
[2] Thermo Fisher Sci, D-28199 Bremen, Germany
[3] Ohio State Univ, Pelotonia Inst Immuno Oncol, Comprehens Canc Ctr, Columbus, OH 43210 USA
[4] Ohio State Univ, Dept Biomed Informat, Columbus, OH 43210 USA
[5] Thermo Fisher Sci, San Jose, CA 95134 USA
基金
美国国家卫生研究院;
关键词
high-resolution mass spectrometry; data-independentacquisition; plasma; quantitative proteomics; SPECTROMETRY;
D O I
10.1021/acs.jproteome.3c00357
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific Orbitrap mass spectrometers, which have long been the gold standard for high-resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high-quality quantitative measurements across a wide dynamic range. We also use a newly developed extracellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5000 plasma proteins in a 60 min gradient with the Orbitrap Astral mass spectrometer.
引用
收藏
页码:3290 / 3300
页数:11
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