Composition and antibacterial, anti-inflammatory, antioxidant, and anticancer activities of Rosmarinus officinalis L. essential oil

被引：15

作者：

Becer, Eda ^{[1
]}

Altundag, Ergul Mutlu ^{[2
]}

Guran, Mumtaz ^{[3
]}

Vatansever, H. Seda ^{[4
,5
]}

Usturk, Selma ^{[6
]}

Hanoglu, Duygu Yigit ^{[7
]}

Baser, K. Husnu Can ^{[8
]}

机构：

[1] Eastern Mediterranean Univ, Fac Pharm, North Cyprus Via Mersin 10, TR-99628 Famagusta, Turkiye

[2] Eastern Mediterranean Univ, Fac Med, Dept Biochem, North Cyprus Via Mersin 10, TR-99628 Famagusta, Turkiye

[3] Eastern Mediterranean Univ, Fac Med, Dept Med Microbiol, North Cyprus Via Mersin 10, TR-99628 Famagusta, Turkiye

[4] Manisa Celal Bayar Univ, Fac Med, Dept Histol & Embryol, Manisa, Turkiye

[5] Near East Univ, DESAM Inst, North Cyprus Via Mersin 10, TR-99138 Nicosia, Turkiye

[6] Eastern Mediterranean Univ, Fac Arts & Sci, Dept Chem, North Cyprus Via Mersin 10, TR-99628 Famagusta, Turkiye

[7] Near East Univ, Fac Pharm, Dept Pharmaceut Bot, North Cyprus Via Mersin 10, TR-99138 Nicosia, Turkiye

[8] Near East Univ, Fac Pharm, Dept Pharmacognosy, North Cyprus Via Mersin 10, TR-99138 Nicosia, Turkiye

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 2023年 / 160卷

关键词：

Rosemary; Essential oil; Cancer; Antibacterial; Antioxidant; HEPATOCELLULAR-CARCINOMA; CHEMICAL-COMPOSITION; CANCER; PLANTS; EXTRACTION; PROGNOSIS; OCT4;

D O I：

10.1016/j.sajb.2023.07.028

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Rosemary is a culinary herb that is widely used in traditional medicine for its nutritional value and pharma-cological properties. The aim of this study was to investigate the composition, in vitro anticancer, antioxi-dant, and antibacterial activities of rosemary essential oil. Rosemary essential oil components were simultaneously analysed by GC/MS and GC/FID techniques. Different concentrations of rosemary essential oil were incubated for 24 and 48 h with HepG2 and EV304 cells. Cell viability was measured by MTT assay. Anti-cancer activities of rosemary essential oil were investigated by immunocytochemistry using antibodies directed against Ki-67, b-catenin, c-myc, Oct-3/4, and IL-8. Anti-inflammatory and antioxidant activity were evaluated with protein denaturation and DPPH assays, respectively. The anti-bacterial effect of rosemary essential oil was analysed by Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae with the broth microdilution and agar zone diffusion methods. Camphor (15.1%), verbenone (14.3%), a-pinene (13.6%), 1,8-cineole (11.8%), and borneol (7.9%) were determined as the major compounds of rosemary essential oil. The activities of the oil were found to be 508.7 mg/ml and 525.7 mg/ml against HepG2 and ECV304 cells, respec-tively. Ki-67, b-catenin, c-myc, Oct-3/4, and IL-8 immunoreactivities were significantly reduced in rosemary essential oil-treated HepG2 cells. Ki-67 and b-catenin immunoreactivities were significantly decreased only in rosemary essential oil-treated ECV304 cells. Also, the essential oil showed antioxidant and free radical scavenging activities. Rosemary essential oil showed effective antibacterial activity on E. coli and K. pneumo-niae. Thus, rosemary essential oil could be a potential candidate as a therapeutic agent in cancer treatment.& COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.

引用

页码：437 / 445

页数：9

共 57 条

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