Protective effects and mechanism of Paecilomyces cicadae TJJ1213 intracellular polysaccharide against H2O2-induced PC12 cells damage

被引：1
|
作者：
Tian, Juanjuan [1 ,2 ]
Xu, Qing [1 ]
Wang, Xiaomeng [1 ]
Zhang, Xueliang [1 ]
Chen, Xiaohong [1 ]
Rui, Xin [1 ]
Zhang, Qiuqin [1 ]
Dong, Mingsheng [1 ]
Li, Wei [1 ,3 ]
机构：
[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Peoples R China
[2] Coll Tea & Food Sci Technol, Jiangsu Vocat Coll Agr & Forestry, Jurong, Peoples R China
[3] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing, Jiangsu, Peoples R China
来源：
CHEMICAL BIOLOGY & DRUG DESIGN | 2023年 / 102卷 / 03期
基金：
中国国家自然科学基金;
关键词：
mitophagy; neuroprotective; oxidative stress; Paecilomyces cicadae TJJ1213; polysaccharide; OXIDATIVE STRESS; AUTOPHAGY; DISEASE; MITOCHONDRIA;
D O I：
10.1111/cbdd.14253
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxidative stress is a key process in the development of neurodegenerative diseases. More attention is needed to screen natural antioxidants and explore pharmacological mechanisms. Natural product polysaccharides with no toxic side effects have powerful antioxidant activity. Two purified intracellular polysaccharide fractions (IPS1 and IPS2) from Paecilomyces cicadae TJJ1213 was isolated. Then, a model of H2O2-induced oxidative stress in PC12 cells was established to investigate the neuroprotective role of IPS and elucidate the potential protection mechanism. Results showed that IPS1 and IPS2 reduced reactive oxygen species (ROS) production, inhibited the leakage of lactate dehydrogenase (LDH) and Ca2+ and attenuated the expression of apoptotic proteins. In addition, western blots displayed that IPS1 and IPS2 significantly inhibited mitophagy induced by H2O2 in PC12 cells via PINK/Parkin pathway. Therefore, IPS1 and IPS2 deserved further investigation as protective agents against neurodegenerative diseases.
引用
收藏
页码：471 / 485
页数：15
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