High-throughput analysis of anthocyanins in horticultural crops using probe electrospray ionization tandem mass spectrometry (PESI/MS/MS)

被引：6

作者：

Ishibashi, Misaki ^{[1
,2
]}

Zaitsu, Kei ^{[3
]}

Yoshikawa, Ikue ^{[1
]}

Otagaki, Shungo ^{[1
,4
]}

Matsumoto, Shogo ^{[1
]}

Oikawa, Akira ^{[2
]}

Shiratake, Katsuhiro ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa, Nagoya, Aichi 4648601, Japan

[2] Kyoto Univ, Grad Sch Agr, Sakyo, Kyoto 6068502, Japan

[3] Kindai Univ, Fac Biol Oriented Sci & Technol, Kinokawa, Wakayama 6496493, Japan

[4] Meijo Univ, Fac Agr, Tenpaku, Nagoya, Aichi 4688502, Japan

来源：

HORTICULTURE RESEARCH | 2023年 / 10卷 / 04期

基金：

日本学术振兴会;

关键词：

ANTIOXIDANT CAPACITY; UNITED-STATES; COMMON FOODS; IDENTIFICATION; MS; PROANTHOCYANIDINS; QUANTIFICATION; METABOLITES; EXTRACTION; QUALITY;

D O I：

10.1093/hr/uhad039

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant secondary metabolites exhibit various horticultural traits. Simple and rapid analysis methods for evaluating these metabolites are in demand in breeding and consumer markets dealing with horticultural crops. We applied probe electrospray ionization (PESI) to evaluate secondary metabolite levels in horticultural crops. PESI does not require pre-treatment and separation of samples, which makes it suitable for high-throughput analysis. In this study, we targeted anthocyanins, one of the primary pigments in horticultural crops. Eighty-one anthocyanins were detected in approximately 3 minutes in the selected reaction-monitoring mode. Tandem mass spectrometry (MS/MS) could adequately distinguish between the fragments of anthocyanins and flavonols. Probe sampling, an intuitive method of sticking a probe directly to the sample, could detect anthocyanins qualitatively on a micro-area scale, such as achenes and receptacles in strawberry fruit. Our results suggest that PESI/MS/MS can be a powerful tool to characterize the profile of anthocyanins and compare their content among cultivars.

引用

页数：10

共 45 条

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