Interaction of Different Fusarium culmorum Isolates and Pratylenchus thornei on Wheat

In present study, the interaction of twelve Fusarium culmorum (W. G. Sm.) Sacc. isolates from Isparta, Burdur in Turkey and Pratylenchus thornei (Sher and Allen),1953 were investigated under controlled conditions (25 +/- 2 degrees C and 60 +/- 5% RH) in Ikizce wheat cultivar. The experiment was established after the first leaves at tillering on wheat and working with four applications. Applications; only P. thornei inoculation (N), only F. culmorum inoculation (FCUL), simultaneous inoculation of P. thornei and F. culmorum (N+FCUL), and R. solani inoculation two weeks after P. thornei application (N+2FCUL) were made. In the inoculation of F. culmorum isolates, 5 ml of 50% diluted culture filtrate concentration was used for each pot, and 1000 larva+adult inoculum density was used for the P. thornei population. Evaluation was carried out 8 weeks after the establishment with disease severity and nematode reproduction rate on wheat. When N+FCUL and N+2FCUL applications were examined in the study, it was observed that P. thornei contributed to the increase of disease severity only in the YLVC16 isolate of F. culmorum. The disease severity scale of YLVC16 isolate was 2.4 in FCUL application, while 3.4 and 3.6 were in N+FCUL and N+2FCUL applications, respectively. The reproduction rate of P. thornei was found to be 2.5 in N application. In the experiment established with YLVC16, T21 and K17 isolates of F. culmorum, the reproduction rate of P. thornei was determined as 3.1, 3.0 and 3.3 in N+FCUL application, while 3.1, 3.2 and 3.4 in N+2FCUL application. Also, in terms of P. thornei reproduction rate, no significant difference was determined between N+FCUL and N+2FCUL applications in the experiment established with YLVC16, T21 and K17 isolates. In the present study, it was determined that only three of the 12 F. culmorum isolates (YLVC16, T21 and K17) contributed positively to the P. thornei reproduction rate, while P. thornei contributed to only one F. culmorum isolate (YLVC16) disease severity.