Biogenic facile green synthesis of actinobacterium exopolysaccharide-fabricated zinc oxide nanoparticles for the diverse biomedical applications

In this investigation, an eco-friendly co-precipitation green routed approach was performed to synthesize zinc oxide (ZnO-NPs) nanoparticles using the exopolysaccharide of an actinobacterial strain. The formation of ZnO-NPs was initially confirmed by peaks of surface plasmon resonance using UV-Vis spectrophotometer. The synthesized nanoparticles were characterized extensively using energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), X-ray diffractometer (XRD), atomic force microscopy (AFM), particle size analyzer (PSA), thermogravimetric analysis (TGA), differential thermal analysis (DTA), and Fourier transform infrared (FTIR) spectroscopy. The produced crystalline nanoparticles were ranged from 18.0 to 40.0 nm. The formed zinc oxide nanoparticles have revealed strong antioxidant potential and also proved their effectiveness against inflammation. ZnO-NPs also exhibited outstanding bactericidal action against clinical pathogenic bacterial strains. Furthermore, the cytotoxicity of nanoparticles was assessed on breast and cervical carcinoma cells. The morphological changes caused in the cancer cells were observed through different fluorescent studies. This synthesis specifies on greener production of ZnO-NPs, and the attained results suggest that it could be applicable for vital therapeutic uses.