Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice

被引：21

作者：

Chen, Dongxia ^{[1
]}

Guo, Junnan ^{[1
]}

Li, Ao ^{[1
]}

Sun, Chengjie ^{[1
]}

Lin, Huibin ^{[2
]}

Lin, Hongyu ^{[1
]}

Yang, Chaoyong ^{[1
,2
]}

Wang, Wei ^{[2
]}

Gao, Jinhao ^{[1
]}

机构：

[1] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem Biol, MOE Key Lab Spectrochem Anal & Instrumentat, Xiamen 361005, Peoples R China

[2] Shanghai Jiao Tong Univ Sch Med, Renji Hosp, Inst Mol Med, Shanghai Key Lab Nucl Acid Chem & Nanomed, Shanghai 200127, Peoples R China

来源：

SCIENCE ADVANCES | 2023年 / 9卷 / 04期

基金：

中国国家自然科学基金;

关键词：

F-19; MRI; INFECTION; TRACKING;

D O I：

10.1126/sciadv.abg6808

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Real-time localization and microbial activity information of indigenous gut microbiota over an extended period of time remains a challenge with existing visualizing methods. Here, we report a metabolic fluorine labeling (MEFLA)-based strategy for monitoring the dynamic gut microbiota via 19F magnetic resonance imaging (19F MRI). In situ labeling of different microbiota subgroups is achieved by using a panel of peptidoglycan-targeting MEFLA probes containing 19F atoms of different chemical shifts, and subsequent real-time in vivo imaging is accomplished by multiplexed hotspot 19F MRI with high sensitivity and unlimited penetration. Using this method, we realize extended visualization (>24 hours) of native gut microbes located at different intestinal sec-tions and semiquantitative analysis of their metabolic dynamics modulated by various conditions, such as the host death and different beta-lactam antibiotics. Our strategy holds great potential for noninvasive and real-time assessing of the metabolic activities and locations of the highly dynamic gut microbiota.

引用

页数：12

共 53 条

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