Three Novel Adenylate Cyclase Genes Show Significant Biological Functions in Plant

被引:14
作者
Liu, Zhiguo [1 ,2 ,3 ]
Yuan, Ye [2 ]
Wang, Lili [1 ]
Zhao, Xuan [1 ,4 ]
Wang, Lixin [2 ]
Wang, Lihu [5 ]
Zhao, Zhihui [1 ]
Zhao, Xin [2 ]
Chu, Yuetong [2 ]
Gao, Yaning
Yang, Fangyuan [2 ]
Wang, Yulu [2 ]
Zhang, Qiong [6 ]
Zhao, Jin [2 ]
Liu, Mengjun [1 ,2 ,3 ]
机构
[1] Hebei Agr Univ, Res Ctr Chinese Jujube, Baoding 071001, Hebei, Peoples R China
[2] Hebei Agr Univ, Coll Hort, Baoding 071001, Hebei, Peoples R China
[3] Jujube Ind Technol Res Inst Hebei, Baoding 071001, Hebei, Peoples R China
[4] Dongzao Res Inst Zhanhua Dist, Binzhou 256800, Shandong, Peoples R China
[5] Hebei Univ Engn, Coll Landscape & Ecol Engn, Handan 056038, Peoples R China
[6] Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
adenylate cyclase; cyclic adenosine monophosphate; Chinese jujube; biological function; regulation mechanism; LONG-TERM-MEMORY; FLOWERING TIME; SEED DORMANCY; 2ND-MESSENGER; EXPRESSION; PATHWAYS; GROWTH; CCA1;
D O I
10.1021/acs.jafc.2c07683
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Adenylate cyclase is the key enzyme solely synthesizing cAMP which participates in cell metabolism regulations and functions as an intracellular second messenger. However, the biological functions of plant ACs have not been elucidated clearly for their poor conservative sequences and low detectable cAMP. We performed a systematic study of plant ACs by using Chinese jujube, whose fruit exhibits the highest cAMP content among plants. Three novel ACs were identified from Chinese jujube, and two types of methods including in vitro and in vivo were used to certificate ZjAC1-3 which can catalyze the conversion of ATP into cAMP. The biological functions of significant accelerations of seed germination, root growth, and flowering were found via overexpression of these AC genes in Arabidopsis, and these functions of ACs were further demonstrated by treating the AC-overexpressing transgenic lines and wild type Arabidopsis with bithionol and dibutyryl-cAMP. At last, transcriptome data revealed that the underlying mechanism of the biological functions of ACs might be regulation of the key genes involved in the circadian rhythm pathway and the hormone signal transduction pathway. This research established a foundation for further investigating plant AC genes and provided strong evidence for cAMP serving as a signaling molecule in plants.
引用
收藏
页码:1149 / 1161
页数:13
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