Integrative Analysis of Multi-Omic Data for the Characteristics of Endometrial Cancer

被引：1

作者：

Li, Tong ^{[1
]}

Ruan, Zhijun ^{[2
]}

Song, Chunli ^{[3
]}

Yin, Feng ^{[3
]}

Zhang, Tuanjie ^{[2
]}

Shi, Liyun ^{[1
]}

Zuo, Min ^{[4
]}

Lu, Linlin ^{[5
]}

An, Yuhao ^{[2
]}

Wang, Rui ^{[2
]}

Ye, Xiyang ^{[1
]}

机构：

[1] Shenzhen Peoples Hosp, Dept Gynecol, Shenzhen 518020, Guangdong, Peoples R China

[2] Pingshan Translat Med Ctr, Shenzhen Bay Lab, Shenzhen 518118, Peoples R China

[3] Peking Univ, Sch Chem Biol & Biotechnol, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China

[4] Shenzhen Peoples Hosp, Dept Pathol, Shenzhen 518020, Guangdong, Peoples R China

[5] Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou 510006, Guangdong, Peoples R China

来源：

ACS OMEGA | 2024年 / 9卷 / 12期

基金：

中国国家自然科学基金;

关键词：

ATYPICAL HYPERPLASIA; PROTEIN; MANAGEMENT; PATHWAY; KINASE;

D O I：

10.1021/acsomega.4c00375

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Endometrial cancer (EC) is a frequently diagnosed gynecologic cancer. Identifying reliable prognostic genes for predicting EC onset is crucial for reducing patient morbidity and mortality. Here, a comprehensive strategy with transcriptomic and proteomic data was performed to measure EC's characteristics. Based on the publicly available RNA-seq data, death-associated protein kinase 3, recombination signal-binding protein for the immunoglobulin kappa J region, and myosin light chain 9 were screened out as potential biomarkers that affect the EC patients' prognosis. A linear model was further constructed by multivariate Cox regression for the prediction of the risk of being malignant. From further integrative analysis, exosomes were found to have a highly enriched role that might participate in EC occurrence. The findings were validated by qRT-polymerase chain reaction (PCR) and western blotting. Collectively, we constructed a prognostic-gene-based model for EC prediction and found that exosomes participate in EC incidents, revealing significantly promising support for the diagnosis of EC.

引用

页码：14489 / 14499

页数：11

共 42 条

[1]

[Anonymous], 2024, A Package for Survival Analysis in R v. R package version 3.7-0

[2] UniProt: the Universal Protein Knowledgebase in 2023 [J].

Bateman, Alex ;

Martin, Maria-Jesus ;

Orchard, Sandra ;

Magrane, Michele ;

Ahmad, Shadab ;

Alpi, Emanuele ;

Bowler-Barnett, Emily H. ;

Britto, Ramona ;

Cukura, Austra ;

Denny, Paul ;

Dogan, Tunca ;

Ebenezer, ThankGod ;

Fan, Jun ;

Garmiri, Penelope ;

Gonzales, Leonardo Jose da Costa ;

Hatton-Ellis, Emma ;

Hussein, Abdulrahman ;

Ignatchenko, Alexandr ;

Insana, Giuseppe ;

Ishtiaq, Rizwan ;

Joshi, Vishal ;

Jyothi, Dushyanth ;

Kandasaamy, Swaathi ;

Lock, Antonia ;

Luciani, Aurelien ;

Lugaric, Marija ;

Luo, Jie ;

Lussi, Yvonne ;

MacDougall, Alistair ;

Madeira, Fabio ;

Mahmoudy, Mahdi ;

Mishra, Alok ;

Moulang, Katie ;

Nightingale, Andrew ;

Pundir, Sangya ;

Qi, Guoying ;

Raj, Shriya ;

Raposo, Pedro ;

Rice, Daniel L. ;

Saidi, Rabie ;

Santos, Rafael ;

Speretta, Elena ;

Stephenson, James ;

Totoo, Prabhat ;

Turner, Edward ;

Tyagi, Nidhi ;

Vasudev, Preethi ;

Warner, Kate ;

Watkins, Xavier ;

Zellner, Hermann .

NUCLEIC ACIDS RESEARCH, 2023, 51 (D1) :D523-D531

[3] Hsp60 Post-translational Modifications: Functional and Pathological Consequences [J].

Bayisotto, Celeste Caruso ;

Alberti, Giusi ;

Vitale, Alessandra Maria ;

Paladino, Letizia ;

Campanella, Claudia ;

Rappa, Francesca ;

Gorska, Magdalena ;

de Macario, Everly Conway ;

Cappello, Francesco ;

Macario, Alberto J. L. ;

Gammazza, Antonella Marino .

FRONTIERS IN MOLECULAR BIOSCIENCES, 2020, 7

[4] The death-associated protein kinases: Structure, function, and beyond [J].

Bialik, Shani ;

Kimchi, Adi .

ANNUAL REVIEW OF BIOCHEMISTRY, 2006, 75 :189-210

[5] mRNAs, proteins and the emerging principles of gene expression control [J].

Buccitelli, Christopher ;

Selbach, Matthias .

NATURE REVIEWS GENETICS, 2020, 21 (10) :630-644

[6] IDENTIFICATION OF A NOVEL SERINE THREONINE KINASE AND A NOVEL 15-KD PROTEIN AS POTENTIAL MEDIATORS OF THE GAMMA-INTERFERON-INDUCED CELL-DEATH [J].

DEISS, LP ;

FEINSTEIN, E ;

BERISSI, H ;

COHEN, O ;

KIMCHI, A .

GENES & DEVELOPMENT, 1995, 9 (01) :15-30

[7] Targeting Wnt Signaling in Endometrial Cancer [J].

Fatima, Iram ;

Barman, Susmita ;

Rai, Rajani ;

Thiel, Kristina W. ;

Chandra, Vishal .

CANCERS, 2021, 13 (10)

[8] Myosin light chain 9 promotes the proliferation, invasion, migration and angiogenesis of colorectal cancer cells by binding to Yes-associated protein 1 and regulating Hippo signaling [J].

Feng, Min ;

Dong, Ningfei ;

Zhou, Xin ;

Ma, Lihong ;

Xiang, Rui .

BIOENGINEERED, 2022, 13 (01) :96-106

[9]

Friedman J., 2021, PACKAGE GLMNET

[10] Integrated genomic characterization of endometrial carcinoma [J].

Getz, Gad ;

Gabriel, Stacey B. ;

Cibulskis, Kristian ;

Lander, Eric ;

Sivachenko, Andrey ;

Sougnez, Carrie ;

Lawrence, Mike ;

Kandoth, Cyriac ;

Dooling, David ;

Fulton, Robert ;

Fulton, Lucinda ;

Kalicki-Veizer, Joelle ;

McLellan, Michael D. ;

O'Laughlin, Michelle ;

Schmidt, Heather ;

Wilson, Richard K. ;

Ye, Kai ;

Ding, Li ;

Mardis, Elaine R. ;

Ally, Adrian ;

Balasundaram, Miruna ;

Birol, Inanc ;

Butterfield, Yaron S. N. ;

Carlsen, Rebecca ;

Carter, Candace ;

Chu, Andy ;

Chuah, Eric ;

Chun, Hye-Jung E. ;

Dhalla, Noreen ;

Guin, Ranabir ;

Hirst, Carrie ;

Holt, Robert A. ;

Jones, Steven J. M. ;

Lee, Darlene ;

Li, Haiyan I. ;

Marra, Marco A. ;

Mayo, Michael ;

Moore, Richard A. ;

Mungall, Andrew J. ;

Plettner, Patrick ;

Schein, Jacqueline E. ;

Sipahimalani, Payal ;

Tam, Angela ;

Varhol, Richard J. ;

Robertson, A. Gordon ;

Pashtan, Itai ;

Saksena, Gordon ;

Onofrio, Robert C. ;

Schumacher, Steven E. ;

Tabak, Barbara .

NATURE, 2013, 497 (7447) :67-73

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