Antioxidant, Cytotoxic, and DNA Damage Protection Activities of Endophytic Fungus Pestalotiopsis neglecta Isolated from Ziziphus spina-christi Medicinal Plant

被引:7
作者
Almustafa, Hibah I. [1 ]
Yehia, Ramy S. [1 ,2 ]
机构
[1] King Faisal Univ, Coll Sci, Dept Biol Sci, Al Hasa 31982, Saudi Arabia
[2] Cairo Univ, Fac Sci, Dept Bot & Microbiol, Giza 12613, Egypt
关键词
bioactive metabolites; endophytic fungi; GC-MS analysis; Pestalotiopsis neglecta; Ziziphus spina-christi; IN-VITRO ANTIOXIDANT; ANTIFUNGAL ACTIVITY; ANTIBACTERIAL ACTIVITIES; ANTIMICROBIAL ACTIVITY; PHYTOCHEMICAL ANALYSIS; NATURAL-PRODUCTS; METABOLITES; LEAVES; CONSTITUENTS; EXTRACTS;
D O I
10.3390/microorganisms11010117
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fungal endophytes are friendly microorganisms that colonize plants and are important in the interactions between plants and their environment. They generate valuable secondary metabolites that are valuable to both plants and humans. Endophytic fungi with bioactivities were isolated from the leaves of the medicinal plant Ziziphus spina-christi. An efficient isolate was selected and identified as Pestalotiopsis neglecta based on nucleotide sequencing of the internal transcribed spacer region (ITS 1-5.8S-ITS 2) of the 18S rRNA gene (NCBI accession number OP529850); the 564 bp had 99 to 100% similarity with P. neglecta MH860161.1, AY682935.1, KP689121.1, and MG572407.1, according to the BLASTn analysis, following preliminary phytochemical and antifungal screening. The biological activities of this fungus' crude ethyl acetate (EtOAc) extract were assessed. With an efficient radical scavenging activity against 2,2 '-diphenyl-1-picrylhydrazyl and an IC50 value of 36.6 mu g mL(-1), P. neglecta extract has shown its potential as an antioxidant. Moreover, it displayed notable cytotoxic effects against MCF-7 (breast carcinoma, IC50 = 22.4 mu g mL(-1)), HeLa (cervical carcinoma, IC50 = 28.9 mu g mL(-1)) and HepG-2 (liver carcinoma, IC50 = 28.9 mu g mL(-1)). At 10 mu g mL(-1), EtOAc demonstrated significant DNA protection against hydroxyl radical-induced damage. Based on FT-IR and GC-MS spectral analysis, it was detected that the EtOAc of P. neglecta product contains multiple bioactive functional groups. Subsequently, this validated the features of major different potent compounds; tolycaine, 1H-pyrazol, 1,3,5-trimethyl-, eugenol, 2,5-cyclohexadiene-1,4-dione, 2,6-bis(1,1-dimethyl), and bis(2-ethylhexyl) phthalate. Since these compounds are biologically relevant in various aspects, and distinct biological activities of fungal extract were acceptable in vitro, this suggests that endophytic fungus P. neglecta may be a viable source of bioactive natural products. This could be a good starting point for pharmaceutical applications.
引用
收藏
页数:19
相关论文
共 96 条
  • [1] Aladesanmi AJ, 2007, AFR J TRADIT COMPLEM, V4, P173
  • [2] Alhakmani Fatma, 2014, Asian Pacific Journal of Tropical Biomedicine, V4, pS656
  • [3] Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori
    Ali S.M.
    Khan A.A.
    Ahmed I.
    Musaddiq M.
    Ahmed K.S.
    Polasa H.
    Rao L.V.
    Habibullah C.M.
    Sechi L.A.
    Ahmed N.
    [J]. Annals of Clinical Microbiology and Antimicrobials, 4 (1)
  • [4] Assessment of farmers practices to date palm soil fertilization and its impact on productivity at Al-Hassa oasis of KSA
    Almadini, Abdulrahman M.
    Ismail, Ahmed I. H.
    Ameen, Faleh A.
    [J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2021, 28 (02) : 1451 - 1458
  • [5] Review: biologically active pyrazole derivatives
    Ansari, Anam
    Ali, Abad
    Asif, Mohd
    Shamsuzzaman
    [J]. NEW JOURNAL OF CHEMISTRY, 2017, 41 (01) : 16 - 41
  • [6] Are tropical fungal endophytes hyperdiverse?
    Arnold, AE
    Maynard, Z
    Gilbert, GS
    Coley, PD
    Kursar, TA
    [J]. ECOLOGY LETTERS, 2000, 3 (04) : 267 - 274
  • [7] Barnett HL., 2006, Illustrated genera of imperfect fungi, V4th
  • [8] Bioactive microbial metabolites -: A personal view
    Bérdy, J
    [J]. JOURNAL OF ANTIBIOTICS, 2005, 58 (01) : 1 - 26
  • [9] Bhardwaj A., 2015, Arch. Clin. Microbiol, V6, P1
  • [10] Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link.
    Bounatirou, S.
    Smiti, S.
    Miguel, M. G.
    Faleiro, L.
    Rejeb, M. N.
    Neffati, M.
    Costa, M. M.
    Figueiredoe, A. C.
    Barroso, J. G.
    Pedro, L. G.
    [J]. FOOD CHEMISTRY, 2007, 105 (01) : 146 - 155