Genetic and Phenotypic Diversity of Listeria monocytogenes in Pig Slaughterhouses in Korea

被引:1
作者
Hong, Serim [1 ]
Moon, Jin-San [2 ]
Yoon, Soon-Seek [2 ]
Kim, Ha-Young [2 ]
Lee, Young Ju [1 ]
机构
[1] Kyungpook Natl Univ, Zoonoses Res Inst, Coll Vet Med, 80 Daehak Ro, Daegu 41566, South Korea
[2] Anim & Plant Quarantine Agcy, Bacterial Dis Div, 177 Hyeoksin 8 Ro, Gimcheon 39660, Gyeongsangbuk D, South Korea
关键词
Listeria monocytogenes; characterization; pigs; carcass; environment; slaughterhouse; ANTIBIOTIC-RESISTANCE; EPIDEMIC CLONES; MULTIPLEX PCR; VIRULENCE; PREVALENCE; CARCASSES; STRAINS; GROWTH;
D O I
10.1089/fpd.2023.0053
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Listeria monocytogenes is a foodborne pathogen that has variable subtypes associated with human listeriosis and occurs in food and processing environments. This study was conducted to provide the genetic and phenotypic characterization of L. monocytogenes in pig carcasses and environments of slaughterhouses in Korea. A total of 22 L. monocytogenes were isolated from eight of 26 pig slaughterhouses between 2020 and 2022, and the most common serotype was 1/2c (40.9%), followed by serotypes 1/2b (31.8%) and 1/2a (27.3%). The isolates showed a significantly high prevalence of virulence genes located in Listeria pathogenicity island-1 (LIPI-1) and internalins (90.9-100%; p < 0.05). However, the prevalence rates of llsX, ptsA, and stress survival islet-1 (SSI-1) located in LIPI-3, LIPI-4, and SSI were only 9.1%, 22.7%, and 31.8%, respectively. In addition, among the epidemic clones (EC), ECI, ECII, ECIII, and ECV, only one isolate was represented as ECV. Isolates identified from the same slaughterhouses were divided into two or more pulsotypes, except for two slaughterhouses. Furthermore, the seven STs were classified into seven clonal complexes (CCs) (CC8, CC9, CC37, CC87, CC121, CC155, and CC288), and all CCs belonged to lineages I (31.8%) and II (68.1%). Interestingly, the isolates showed a high prevalence of oxacillin resistance (59.1%), and most isolates of the serotypes 1/2a and 1/2b exhibited oxacillin resistance, whereas only one of nine serotype 1/2c isolates exhibited oxacillin resistance. These results provide the genetic diversity of L. monocytogenes in pig carcasses and environments of slaughterhouses, and continuous monitoring will be helpful in predicting food safety risks.
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页码:1 / 9
页数:9
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