Propolis nanoparticles synthesis and characterization with cytotoxic and apoptotic effects on breast cancer cells

被引：7

作者：

Ay, Ebru Nur ^{[1
]}

Caner, Armagan ^{[2
]}

Hepokur, Ceylan Ozsoy ^{[3
]}

Kalindemirtas, Ferdane Danisman ^{[4
]}

Eroglu, Gunes Ozen ^{[5
]}

Kariper, I. Afsin ^{[6
]}

机构：

[1] Istinye Univ, Vocat Sch Hlth Serv, Istanbul, Turkiye

[2] Erciyes Univ, Fac Med, Dept Biophys, Kayseri, Turkiye

[3] Cumhuriyet Univ, Fac Pharm, Dept Biochem, Sivas, Turkiye

[4] Erzincan Binali Yildirim Univ, Fac Med, Dept Physiol, Erzincan, Turkiye

[5] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Mol Med, Istanbul, Turkiye

[6] Erciyes Univ, Fac Educ, Kayseri, Turkiye

来源：

JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE | 2023年 / 17卷 / 01期

关键词：

Breast cancer; propolis; nanoparticle; cytotoxicity; ACID PHENETHYL ESTER; CYCLE ARREST; GROWTH; NANOSTRUCTURES; ANTIOXIDANT; INHIBITION; DELIVERY;

D O I：

10.1080/16583655.2023.2249628

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this work was to investigate the antiproliferative and pro-apoptotic effects of propolis on human breast cancer cells using a simple and inexpensive nanoformulation. Various methods were used to characterize the surface and morphology of propolis nanoparticles (NP-Pro), including UV-VIS, FTIR, DLS, and EDX-SEM. The cytotoxic activity of MCF-7, MDA-MB-231 breast cancer cells was measured by XTT technique and compared with healthy cells (MCF-10A). The apoptotic effect of NP-Pro was assessed by flow cytometry using Annexin/PI. The IC50 values of NP-Pro on MCF-7, MDA-MB-231 and MCF-10A cells were 13.67 +/- 0.89, 17.89 +/- 0.6, 29.9 +/- 0.56 mu g, respectively. According to these findings, the NP-Pro form we prepared was more cytotoxic on cancer cells than propolis. Cancer cells had much stronger apoptotic activity than healthy cells (p < 0.0001). In this work, NP-Pro was produced through a distinct, straightforward, and low-cost method. In summary, the NP-Pro we synthesized may be a promising potential agent for breast cancer treatment due to its specific cytotoxicity via apoptosis.

引用

页数：11

共 57 条

[11] Polymeric Nanoparticles of Brazilian Red Propolis Extract: Preparation, Characterization, Antioxidant and Leishmanicidal Activity [J].