Development of a sensitive HPLC method with fluorescence detection for determination of motilin in human plasma

被引：2

作者：

Karaca, Sakine Atila ^{[1
]}

Temel, Tuncer ^{[2
]}

Ugur, Duygu Yeniceli ^{[1
]}

机构：

[1] Anadolu Univ, Fac Pharm, Dept Analyt Chem, TR-26470 Eskisehir, Turkiye

[2] Eskisehir Osmangazi Univ, Fac Med, Dept Internal Med, TR-26040 Eskisehir, Turkiye

来源：

BIOANALYSIS | 2023年 / 15卷 / 04期

关键词：

chromatography; experimental design; motilin; peptide; quantification; LIQUID-CHROMATOGRAPHY; PEPTIDES; GHRELIN;

D O I：

10.4155/bio-2023-0003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Motilin is a peptide-structured gastrointestinal system hormone. In this study, a sensitive HPLC-fluorescence detection method was developed and validated for the quantification of motilin in human plasma. Materials & methods: Optimization processes were carried out with the experimental design methodology. Analyses were performed on a C8 column (4.6 x 150 mm, 3.5 mu m particles) using water and acetonitrile containing trifluoroacetic acid as the mobile phase. Results & conclusion: The method was linear from 2 to 200 ng/ml of motilin. The assay variability was less than 5%. The limit of quantification was found to be 1.84 ng/ml. The applicability of the developed method was successfully demonstrated by quantifying the levels of motilin in human plasma samples.

引用

页码：219 / 229

页数：11

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