Insight into biological activities of chemically characterized extract from Marrubium vulgare L. in vitro, in vivo and in silico approaches

被引：6

作者：

Gourich, Aman Allah ^{[1
]}

Touijer, Hanane ^{[1
]}

Drioiche, Aziz ^{[1
]}

Asbabou, Ayoub ^{[1
]}

Remok, Firdaous ^{[1
]}

Saidi, Soukaina ^{[1
]}

Siddique, Farhan ^{[2
]}

Ailli, Atika ^{[1
]}

Bourhia, Mohammed ^{[3
]}

Salamatullah, Ahmad Mohammad ^{[4
]}

Ouahmane, Lahcen ^{[5
]}

Mouradi, Aicha ^{[1
]}

Eto, Bruno ^{[6
]}

Zair, Touriya ^{[1
]}

机构：

[1] Moulay Ismail Univ, Fac Sci, Lab Innovat Mat & Biotechnol Nat Resources, Res Team Chem Bioact Mol & Environm, Meknes, Morocco

[2] Bahauddin Zakariya Univ, Fac Pharm, Dept Pharmaceut Chem, Multan, Pakistan

[3] Ibn Zohr Univ, Fac Med & Pharm, Dept Chem & Biochem, Agadir, Morocco

[4] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Riyadh, Saudi Arabia

[5] Cadi Ayyad Univ, Labeled Res Unit CNRSTN 4, Lab Microbial Biotechnol Agrosci & Environm BioMAg, Marrakech, Morocco

[6] Univ Lille, Fac Pharm, Lab Pharmacol Pharmacokinet & Clin Pharm, Labs TBC, F-59000 Lille, France

来源：

FRONTIERS IN CHEMISTRY | 2023年 / 11卷

关键词：

Marrubium vulgare L; polyphenols; LC/MS; antioxidant; toxicity; antimitotic; antimicrobial; antidiabetic; ACUTE MYOCARDIAL-INFARCTION; ANTIOXIDANT ACTIVITY; MEDICINAL-PLANTS; PHENOLIC-COMPOUNDS; ANTIMICROBIAL ACTIVITIES; METHANOLIC EXTRACT; ESSENTIAL OILS; ALPHA-AMYLASE; DRUG DESIGN; POLYPHENOLS;

D O I：

10.3389/fchem.2023.1238346

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Aqueous extracts of Marrubium vulgare L. (M. vulgare) are widely used in traditional medicine for their therapeutic effects. Hence, this study aims to evaluate in vitro, in vivo, and in silico the biological activities of M. vulgare aqueous extract to further support their traditional use. Qualitative phytochemical tests of M. vulgare extracts showed the presence of primary and secondary metabolites, while quantitative analyses recorded revealed the contents of total phenols, flavonoids, and tannins, with values of 488.432 +/- 7.825 mg/EAG gallic acid extract/g, 25.5326 +/- 1.317 mg/EQ Quercetin extract/g and 23.966 +/- 0.187 mg/EC catechin extract/g, respectively. Characterization of the phytochemical constituents of the extract revealed the presence of catechin and maleic acid as the most abundant while the evaluation of the antioxidant power revealed that the extract possesses significant antioxidant capacity, antimitotic potential, and antimicrobial properties against Streptococcus agalactiae and Staphylococcus epidermidis among many others. The antidiabetic activity of the extract showed a potent antihyperglycemic effect and a significant modulation of the pancreatic a-amylase activity as revealed by both in vitro and in vivo analysis, while an in silico evaluation showed that chemicals in the studied extract exhibited the aforementioned activities by targeting 1XO2 antimitotic protein, W93 antidiabetic protein and 1AJ6 antimicrobial protein, which revealed them as worthy of exploration in drug discovery odyssey. Conclusively, the result of this study demonstrates the numerous biological activities of M. vulgare and gives credence to their folkloric and traditional usage.

引用

页数：19

共 92 条

[1]

1AJ6, 2023, NOVOBIOCIN-RESISTANT mutant (R136H) of the N-terminal 24 kda fragment of dna gyrase B complexed with NOVOBIOCIN at 2.3 angstroms resolution

[2]

1XO2, 2023, Crystal structure of a human cyclin-dependent kinase 6 complex with a flavonol inhibitor, fisetin

[3]

4W93, 2023, Human pancreatic alpha-amylase in complex with montbretin A

[4]

AFNOR, 1972, NF V05-113. Fruits

[5]

AFNOR, 1977, Aliments des animaux. Dosage des cendres brute, pV18

[6]

AFNOR, 1985, NF V03-402. Epices et aromates - Determination de la teneur en eau - Methode par entrainement

[7] Phytochemical analysis and assessment of antioxidant and antimicrobial activities of some medicinal plant species from Egyptian flora [J].