Rewiring Bacillus subtilis and bioprocess optimization for oxidoreductive reaction-mediated biosynthesis of D-tagatose

被引:7
|
作者
Zhang, Guoyan [1 ]
An, Yingfeng [2 ]
Zabed, Hossain M. [3 ]
Yun, Junhua [3 ]
Parvez, Amreesh [4 ]
Zhao, Mei [1 ]
Zhang, Cunsheng [1 ]
Ravikumar, Yuvaraj [1 ]
Li, Jia [3 ]
Qi, Xianghui [1 ,3 ]
机构
[1] Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China
[2] Shenyang Agr Univ, Coll Biosci & Biotechnol, 120 Dongling Rd, Shenyang 110161, Liaoning, Peoples R China
[3] Guangzhou Univ, Sch Mech & Elect Engn, 230 Wai Huan Xi Rd, Guangzhou 510006, Guangdong, Peoples R China
[4] Univ Western Cape, Inst Microbial Biotechnol & Metagen, Robert Sobukwe Rd, ZA-7535 Bellville, South Africa
基金
中国国家自然科学基金;
关键词
D-tagatose; Oxidoreductase reaction; Enzyme identification; Metabolic engineering; Fermentation optimization; CHEESE WHEY;
D O I
10.1016/j.biortech.2023.129843
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
D-tagatose holds significant importance as a functional monosaccharide with diverse applications in food, medicine, and other fields. This study aimed to explore the oxidoreductive pathway for D-tagatose production, surpassing the contemporary isomerization-mediated biosynthesis approach in order to enhance the thermodynamic equilibrium of the reactions. Initially, a novel galactitol dehydrogenase was discovered through biochemical and bioinformatics analyses. By co-expressing the galactitol dehydrogenase and xylose reductase, the oxidoreductive pathway for D-tagatose synthesis was successfully established in Bacillus subtilis. Subsequently, pathway fine-tuning was achieved via promoter regulation and dehydrogenase-mediated cofactor regeneration, resulting in 6.75-fold higher D-tagatose compared to that produced by the strain containing the unmodified promoter. Finally, optimization of fermentation conditions and medium composition produced 39.57 g/L D-tagatose in a fed-batch experiment, with a productivity of 0.33 g/L/h and a yield of 0.55 mol/mol D-galactose. These findings highlight the potential of the constructed redox pathway as an effective approach for D-tagatose production.
引用
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页数:10
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