Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids

被引：46

作者：

Ai, Zongyong ^{[1
,2
,3
]}

Niu, Baohua ^{[1
,2
]}

Yin, Yu ^{[1
,2
,3
]}

Xiang, Lifeng ^{[4
]}

Shi, Gaohui ^{[1
,2
]}

Duan, Kui ^{[1
,2
,3
]}

Wang, Sile ^{[1
,2
,3
]}

Hu, Yingjie ^{[1
,2
]}

Zhang, Chi ^{[1
,2
]}

Zhang, Chengting ^{[1
,2
,3
]}

Rong, Lujuan ^{[1
,2
]}

Kong, Ruize ^{[4
]}

Chen, Tingwei ^{[1
,2
]}

Guo, Yixin ^{[5
]}

Liu, Wanlu ^{[5
]}

Li, Nan ^{[1
,2
]}

Zhao, Shumei ^{[1
,2
,3
]}

Zhu, Xiaoqing ^{[1
,2
,3
]}

Mai, Xuancheng ^{[4
]}

Li, Yonggang ^{[4
]}

Wu, Ze ^{[4
]}

Zheng, Yi ^{[6
]}

Fu, Jianping ^{[6
,7
,8
]}

Ji, Weizhi ^{[1
,2
,3
]}

Li, Tianqing ^{[1
,2
,3
]}

机构：

[1] Kunming Univ Sci & Technol, Inst Primate Translat Med, State Key Lab Primate Biomed Res, Kunming, Yunnan, Peoples R China

[2] Yunnan Key Lab Primate Biomed Res, Kunming, Yunnan, Peoples R China

[3] Yunnan Prov Acad Sci & Technol, Kunming, Yunnan, Peoples R China

[4] First Peoples Hosp Yunnan Prov, Dept Reprod Med, Kunming, Yunnan, Peoples R China

[5] Zhejiang Univ, Univ Edinburgh Inst, Sch Med, Int Campus,ZJU UoE Inst, Hangzhou, Zhejiang, Peoples R China

[6] Univ Michigan, Dept Mech Engn, Ann Arbor, MI USA

[7] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI USA

[8] Univ Michigan, Med Sch, Dept Cell & Dev Biol, Ann Arbor, MI USA

来源：

CELL RESEARCH | 2023年 / 33卷 / 09期

基金：

中国国家自然科学基金;

关键词：

PLURIPOTENT STEM-CELLS; NAIVE PLURIPOTENCY; CLINICAL TRANSLATION; SELF-ORGANIZATION; STATE; MODEL; DIFFERENTIATION; IDENTIFICATION; ESTABLISHMENT; IMPLANTATION;

D O I：

10.1038/s41422-023-00846-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Studies of cultured embryos have provided insights into human peri-implantation development. However, detailed knowledge of peri-implantation lineage development as well as underlying mechanisms remains obscure. Using 3D-cultured human embryos, herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives. In addition, we develop an embryo-like assembloid (E-assembloid) by assembling naive hESCs and extraembryonic cells. Using human embryos and E-assembloids, we reveal that WNT, BMP and Nodal signaling pathways synergistically, but functionally differently, orchestrate human peri-implantation lineage development. Specially, we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications. Finally, an improved E-assembloid is developed to recapitulate the epiblast and hypoblast development and tissue architectures in the pre-gastrulation human embryo. Our findings provide insights into human peri-implantation development, and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis.

引用

页码：661 / 678

页数：18

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