Genotoxic effects of occupational exposure to glass fibres-A human biomonitoring study

被引:6
作者
Ceppi, Marcello [1 ]
Smolkova, Bozena
Staruchova, Marta [3 ]
Kazimirova, Alena [3 ]
Barancokova, Magdalena [3 ]
Volkovova, Katarina [3 ]
Collins, Andrew [4 ]
Kocan, Anton [3 ]
Dzupinkova, Zuzana [3 ,5 ]
Horska, Alexandra [3 ]
Buocikova, Verona [2 ]
Tulinska, Jana [6 ]
Liskova, Aurelia [6 ]
Mikusova, Miroslava Lehotska [6 ]
Krivosikova, Zora [7 ]
Wsolova, Ladislava [8 ]
Kuba, Daniel [9 ]
Runden-Pran, Elise [10 ]
El Yamani, Naouale [10 ]
Longhin, Eleonora Martha [10 ]
Halasova, Erika [11 ]
Kyrtopoulos, Soterios [12 ]
Bonassi, Stefano [13 ]
Dusinska, Maria [10 ]
机构
[1] San Martino Policlin Hosp, Biostat Unit, Genoa, Italy
[2] Slovak Acad Sci, Canc Res Inst, Biomed Res Ctr, Bratislava, Slovakia
[3] Slovak Med Univ, Fac Med, Dept Biol, Bratislava, Slovakia
[4] Univ Oslo, Inst Basic Med Sci, Dept Nutr, Oslo, Norway
[5] Inst Mol Biotechnol Austrian Acad Sci IMBA, Vienna Bioctr VBC, Vienna, Austria
[6] Slovak Med Univ, Lab Immunotoxicol, Bratislava, Slovakia
[7] Slovak Med Univ, Dept Clin & Expt Pharmacotherapy, Bratislava, Slovakia
[8] Slovak Med Univ, Inst Biophys Informat & Biostat, Fac Publ Hlth, Bratislava, Slovakia
[9] Natl Transplant Org, Bratislava, Slovakia
[10] NILU Norwegian Inst Air Res, Hlth Effects Lab, Dept Environm Chem, Kjeller, Norway
[11] Comenius Univ, Jessenius Fac Med Martin, Biomed Ctr Martin, Martin, Slovakia
[12] Natl Hellen Res Fdn, Inst Biol Med Chem & Biotechnol, Athens, Greece
[13] IRCCS San Raffaele Pisana, Unit Clin & Mol Epidemiol, Rome, Italy
关键词
Glass fibre; Occupational exposure; Human biomonitoring; Biomarkers; DNA instability; Genetic polymorphism; Immunomodulatory markers; GLUTATHIONE S-TRANSFERASES; COMET ASSAY; DNA-DAMAGE; MINERAL FIBERS; IN-VITRO; MICRONUCLEUS; REPAIR; ASBESTOS;
D O I
10.1016/j.mrgentox.2022.503572
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
As part of a large human biomonitoring study, we conducted occupational monitoring in a glass fibre factory in Slovakia. Shopfloor workers (n = 80), with a matched group of administrators in the same factory (n = 36), were monitored for exposure to glass fibres and to polycyclic aromatic hydrocarbons (PAHs). The impact of occu-pational exposure on chromosomal aberrations, DNA damage and DNA repair, immunomodulatory markers, and the role of nutritional and lifestyle factors, as well as the effect of polymorphisms in metabolic and DNA repair genes on genetic stability, were investigated.The (enzyme-modified) comet assay was employed to measure DNA strand breaks (SBs) and apurinic sites, oxidised and alkylated bases. Antioxidant status was estimated by resistance to H2O2-induced DNA damage. Base excision repair capacity was measured with an in vitro assay (based on the comet assay).Exposure of workers to fibres was low, but still was associated with higher levels of SBs, and SBs plus oxidised bases, and higher sensitivity to H2O2. Multivariate analysis showed that exposure increased the risk of high levels of SBs by 20%. DNA damage was influenced by antioxidant enzymes catalase and glutathione S-transferase (measured in blood). DNA repair capacity was inversely correlated with DNA damage and positively with antioxidant status. An inverse correlation was found between DNA base oxidation and the percentage of eosinophils (involved in the inflammatory response) in peripheral blood of both exposed and reference groups. Genotypes of XRCC1 variants rs3213245 and rs25487 significantly decreased the risk of high levels of base oxidation, to 0.50 (p = 0.001) and 0.59 (p = 0.001), respectively.Increases in DNA damage owing to glass fibre exposure were significant but modest, and no increases were seen in chromosome aberrations or micronuclei. However, it is of concern that even low levels of exposure to these fibres can cause significant genetic damage.
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页数:10
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