A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function

被引：5
作者：
Han, Xiuxin [1 ]
Yin, Mengfan [1 ,2 ]
Gong, Chen [3 ]
Zhang, Chao [1 ]
Zhu, Genbao [3 ]
Hu, Mengxue [3 ]
Tan, Kemeng [3 ]
Jiang, La [3 ]
Wang, Guowen [1 ,5 ]
Li, Lili [3 ,4 ]
机构：
[1] Tianjin Med Univ Canc Inst & Hosp, Natl Clin Res Ctr Canc, Tianjins Clin Res Ctr Canc, Key Lab Canc Prevent & Therapy, Tianjin, Peoples R China
[2] Tianjin Fifth Cent Hosp, Dept Orthoped Surg, Tianjin, Peoples R China
[3] Anhui Wanbei Coal Elect Grp Gen Hosp, Gen Clin Res Ctr, Suzhou, Anhui, Peoples R China
[4] Anhui Wanbei Coal Elect Grp Gen Hosp, 125 Huaihe West Rd, Suzhou 234000, Anhui, Peoples R China
[5] Tianjin Med Univ Canc Inst & Hosp, Dept Bone & Soft Tissue Tumor, 24 Huanhu West Rd, Tianjin 300060, Peoples R China
来源：
EXPERT OPINION ON THERAPEUTIC TARGETS | 2023年 / 27卷 / 10期
关键词：
Osteosarcoma; long non-coding RNA A1BG antisense RNA 1; microRNA-148a-3p; ubiquitin-specific protease 22; silent information regulator 2 homolog 1; deubiquitination; HEPATOCELLULAR-CARCINOMA; PROLIFERATION; INVASION; CANCER; SUPPRESSES;
D O I：
10.1080/14728222.2023.2263908
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
BackgroundThe study aims to explore the role of A1BG antisense RNA 1 (A1BG-AS1), microRNA (miR)-148a-3p and ubiquitin-specific protease 22 (USP22) on osteosarcoma (OS) cell growth.Research design & methodsA1BG-AS1, miR-148a-3p, USP22, and silent information regulator 2 homolog 1 (SIRT1) levels in OS tissues and cells were determined. The effects of A1BG-AS1, miR-148a-3p, and USP22 on the biological functions of OS cells were examined by functional assays. In vivo assay was conducted to observe the effect of A1BG-AS1 on OS growth in vitro. The relationship of A1BG-AS1, miR-148a-3p, and USP22 was analyzed by bioinformatics analysis, RNA-fluorescence in situ hybridization, luciferase activity, and RNA binding protein immunoprecipitation assays. The relation between USP22 and SIRT1 was evaluated by immunoprecipitation.ResultsA1BG-AS1 and USP22 were highly expressed, and miR-148a-3p was lowly expressed in OS tissues and cells. Down-regulation of A1BG-AS1 and USP22 or up-regulation of miR-148a-3p impaired the malignant behaviors of OS cells. A1BG-AS1 sponged miR-148a-3p, and miR-148a-3p targeted USP22, thereby inhibiting USP22 expression. Up-regulating USP22 reversed the A1BG-AS1 suppression-induced phenotypic inhibition of OS cells. USP22 affected the biological functions of OS cells by deubiquitinating SIRT1.ConclusionA1BG-AS1 facilitates the biological functions of OS cells via mediating the miR-148a-3p/USP22 axis.
引用
收藏
页码：1017 / 1029
页数：13
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