Arbuscular mycorrhizal fungi inoculation impacts expression of aquaporins and salt overly sensitive genes and enhances tolerance of salt stress in tomato

被引:21
作者
Liu, Ming-Yang [1 ]
Li, Qiu-Shuang [1 ]
Ding, Wan-Yu [1 ]
Dong, Li-Wei [1 ]
Deng, Min [1 ]
Chen, Jia-Hui [1 ]
Tian, Xiao [1 ]
Hashem, Abeer [2 ]
Al-Arjani, Al-Bandari Fahad [2 ]
Alenazi, Mekhled M. [3 ]
Abd-Allah, Elsayed Fathi [3 ]
Wu, Qiang-Sheng [1 ]
机构
[1] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China
[2] King Saud Univ, Coll Sci, Bot & Microbiol Dept, POB 2460, Riyadh 11451, Saudi Arabia
[3] King Saud Univ, Coll Food & Agr Sci, Plant Prod Dept, POB 2460, Riyadh 11451, Saudi Arabia
关键词
Arbuscular mycorrhizal fungi; PIPs; Salt stress; SOS; TIPs; PHYSIOLOGICAL-RESPONSE; TRIFOLIATE ORANGE; SALINITY; PLANTS; SYMBIOSIS;
D O I
10.1186/s40538-022-00368-2
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Arbuscular mycorrhizal fungi (AMF) that establish reciprocal symbiosis with plant roots can enhance resistance to various stresses, including salt stress, but relevant mechanisms, especially at the molecular level, are scarce. The objective of this study was to analyze the effect of an arbuscular mycorrhizal fungus Paraglomus occultum on plant growth, leaf gas exchange, and expression of plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs) and salt overly sensitive (SOS) genes in tomato under salt (150 mmol/L NaCl) and non-salt stress. Salt stress for 4 weeks inhibited root colonization rate of P. occultum and soil hyphal length by 0.21- and 0.57-fold, respectively. Salt stress also inhibited plant growth performance and leaf gas exchange, while inoculation with P. occultum significantly enhanced them under salt and non-salt stress conditions. AMF showed diverse regulation of root SlPIPs and SlTIPs expression, among which under salt stress, SlPIP1;2, SlPIP1;5, SlPIP2;1, SlPIP2;6, SlPIP2;9, SlPIP2;10, SlTIP2;2, SlTIP3;2, and SlTIP5;1 were up-regulated by AMF colonization, and SlPIP1;7, SlPIP2;5, SlPIP2;8, SlPIP2;11, SlPIP2;12, SlTIP2;3, and SlTIP3;1 were down-regulated, accompanied by no change in SlPIP1;1, SlPIP1;3, SlPIP2;4, SlTIP1;1, SlTIP1;2, SlTIP1;3, SlTIP2;1, and SlTIP2;5. Interestingly, salt stress inhibited the expression of SlSOS1 and SlSOS2 in non-mycorrhizal plants, while it increased the expression of SlSOS1 and SlSOS2 in mycorrhizal plants. AMF colonization down-regulated expression of SlSOS1 and SlSOS2 under non-salt stress while up-regulated expression of SlSOS1 and SlSOS2 under salt stress. It was concluded that AMF inoculation impacted the expression of stress-responsive genes, especially SOS1 and SOS2, and enhanced salt resistance of tomato.
引用
收藏
页数:11
相关论文
共 40 条
  • [1] Arbuscular mycorrhizal symbiosis regulates the physiological responses, ion distribution and relevant gene expression to trigger salt stress tolerance in pistachio
    Abbaspour, Hossein
    Pour, Fatemeh S. N.
    Abdel-Wahhab, Mosaad A.
    [J]. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2021, 27 (08) : 1765 - 1778
  • [2] Effectiveness of arbuscular mycorrhizal fungi (AMF) for inducing the accumulation of major carotenoids, chlorophylls and tocopherol in green and red leaf lettuces
    Baslam, Marouane
    Esteban, Raquel
    Garcia-Plazaola, Jose I.
    Goicoechea, Nieves
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 97 (07) : 3119 - 3128
  • [3] Bertolazi AA, 2018, MICROORG SUSTAIN, V5, P351, DOI 10.1007/978-981-10-5514-0_16
  • [4] COMPARISON OF 2 METHODS FOR QUANTIFYING EXTRARADICAL MYCELIUM OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI
    BETHLENFALVAY, GJ
    AMES, RN
    [J]. SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1987, 51 (03) : 834 - 837
  • [5] Bhardwaj R., 2013, ECOPHYSIOLOGY RESPON, P2139
  • [6] Arbuscular Mycorrhizal Symbiosis Alleviates Salt Stress in Black Locust through Improved Photosynthesis, Water Status, and K+/Na+ Homeostasis
    Chen, Jie
    Zhang, Haoqiang
    Zhang, Xinlu
    Tang, Ming
    [J]. FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [7] Plant Aquaporin Responses to Mycorrhizal Symbiosis under Abiotic Stress
    Cheng, Hui-Qian
    Ding, Yu-E
    Shu, Bo
    Zou, Ying-Ning
    Wu, Qiang-Sheng
    Kuca, Kamil
    [J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, 2020, 23 (04) : 786 - 794
  • [8] Mycorrhizal response strategies of trifoliate orange under well-watered, salt stress, and waterlogging stress by regulating leaf aquaporin expression
    Cheng, Xiao-Fen
    Wu, Hui-Hui
    Zou, Ying-Ning
    Wu, Qiang-Sheng
    Kuca, Kamil
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2021, 162 : 27 - 35
  • [9] Effects of mycorrhizas on physiological performance and root TIPs expression in trifoliate orange under salt stress
    Ding, Yu-E
    Fan, Qing-Feng
    He, Jia-Dong
    Wu, Hui-Hui
    Zou, Ying-Ning
    Wu, Qiang-Sheng
    Kuca, Kamil
    [J]. ARCHIVES OF AGRONOMY AND SOIL SCIENCE, 2020, 66 (02) : 182 - 192
  • [10] Dong Chao, 2016, Plant Science Journal, V34, P99, DOI 10.11913/PSJ.2095.0837.2016.10099