Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

被引：5
作者：
Hao, ZengTao [1 ]
Yin, Chao [1 ]
Wang, XiaoLong [1 ]
Huo, ZhiQi [1 ]
Zhang, GuoRong [1 ]
Jiang, Dong [1 ]
An, Min [1 ,2 ]
机构：
[1] Inner Mongolia Med Univ, Affiliated Hosp 2, Dept Hand & Foot Microsurg, 59 Horqin Rd, Hohhot 010000, Peoples R China
[2] Inner Mongolia Med Univ, Affiliated Hosp 2, Dept Anesthesiol, 59 Horqin Rd, Hohhot 010000, Peoples R China
来源：
HELIYON | 2023年 / 9卷 / 11期
基金：
美国国家卫生研究院;
关键词：
Tetramethylpyrazine; miR-497-5p; EGFL7; Angiogenesis; Spinal cord injury; Nerve regeneration; Nerve defect repair; OXYGEN-GLUCOSE DEPRIVATION; VASCULAR DISRUPTION; FUNCTIONAL RECOVERY; DOWN-REGULATION; EXPRESSION; MICRORNAS; CELL; APOPTOSIS; NEURONS; BIOLOGY;
D O I：
10.1016/j.heliyon.2023.e21549
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Objective: This study evaluated the regulatory effect of Tetramethylpyrazine (TMP) on the spinal cord injury (SCI) rat model and clarified the neuroprotective mechanism of TMP on SCI. Methods: An SCI rat model was generated and treated with TMP injections for two weeks. miR497-5p and EGFL7 expression changes were evaluated, motor function recovery after SCI was assessed by BBB score test and footprint analysis, lesions of rat spinal cord were assessed by HE staining and TUNEL staining; angiogenesis was assessed by immunoblotting for CD31; inflammatory factor levels were detected by ELISA. EGFL7 was verified as a target of miR-497-5p by bioinformatics website analysis and luciferase reporter gene assay. H2O2-injured neurons were cultured in vitro to explore the effect of TMP.Results: After SCI, miR-497-5p was upregulated while EGFL7 was downregulated in rats. TMP inhibited apoptosis and promoted angiogenesis, nerve regeneration, and repair of nerve defects by reducing miR-497-5p and increasing EGFL7 expression. miR-497-5p targeted EGFL7. In addition, TMP hindered neuronal inflammation and apoptosis induced by H2O2 in vitro.Conclusion: TMP promotes angiogenesis by downregulating miR-497-5p to target EGFL7, and promotes nerve regeneration and repair of nerve defects in rats with SCI.
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页数：11
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