Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

被引:13
作者
Diba, Farzana [1 ,2 ]
Hoque, M. Nazmul [3 ]
Rahman, M. Shaminur [4 ]
Haque, Farhana [1 ]
Rahman, Khondaker Md. Jaminur [5 ]
Moniruzzaman, Md. [6 ]
Khan, Mala [6 ]
Hossain, M. Anwar [1 ,7 ]
Sultana, Munawar [1 ]
机构
[1] Univ Dhaka, Dept Microbiol, Dhaka 1000, Bangladesh
[2] Atom Energy Res Estab, Inst Tissue Banking & Biomat Res, Dhaka 1349, Bangladesh
[3] Bangabandhu Sheikh Mujibur Rahman Agr Univ, Dept Gynecol Obstet & Reprod Hlth, Gazipur, Bangladesh
[4] Jashore Univ Sci & Technol, Dept Microbiol, Jashore 7408, Bangladesh
[5] Natl Inst Biotechnol, Environm Sci Div, Dhaka 1349, Bangladesh
[6] Bangladesh Reference Inst Chem Measurements BRiCM, Dr Kudrat i Khuda Rd, Dhanmondi, Dhaka, Bangladesh
[7] Jashore Univ Sci & Technol, Jashore 7408, Bangladesh
关键词
Arsenotrophic bacteria; Arsenotrophic genes; Whole-genome shotgun sequencing; AMRs; VFGs; Bioremediation; CONTAMINATED GROUNDWATER; ANTIBIOTIC-RESISTANCE; OXIDASE; DIVERSITY; BACTERIUM;
D O I
10.1186/s12866-023-02980-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background Arsenic (As) and its species are major pollutants in ecological bodied including groundwater in Bangladesh rendering serious public health concern. Bacteria with arsenotrophic genes have been found in the aquifer, converting toxic arsenite [As (III)] to less toxic arsenate [As (V)] that is easily removed using chemical and biological trappers. In this study, genomic and metagenomic approaches parallel to culture-based assay (Graphical abstract) have made it possible to decipher phylogenetic diversity of groundwater arsenotrophic microbiomes along with elucidation of their genetic determinants.Results Seventy-two isolates were retrieved from six As-contaminated (average As concentration of 0.23 mg/L) groundwater samples from Munshiganj and Chandpur districts of Bangladesh. Twenty-three isolates harbored arsenite efflux pump (arsB) gene with high abundance, and ten isolates possessing arsenite oxidase (aioA) gene, with a wide range of minimum inhibitory concentration, MICAs (2 to 32 mM), confirming their role in arsenite metabolism. There was considerable heterogeneity in species richness and microbial community structure. Microbial taxa from Proteobacteria, Firmicutes and Acidobacteria dominated these diversities. Through these combinatorial approaches, we have identified potential candidates such as, Pseudomonas, Acinetobacter, Stenotrophomonas, Achromobacter, Paraburkholderia, Comamonas and Klebsiella and associated functional genes (arsB, acr3, arsD, arsH, arsR) that could significantly contribute to arsenite detoxification, accumulation, and immobilization.Conclusions Culture-dependent and -independent shotgun metagenomic investigation elucidated arsenotrophic microbiomes and their functions in As biogeochemical transformation. These findings laid a foundation for further large-scale researches on the arsenotrophic microbiomes and their concurrent functions in As biogeochemical transformation in As-contaminated areas of Bangladesh and beyond.
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页数:20
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