Umbelliferone loaded PEGylated liposomes: preparation, characterization and its mitigatory effects on Dalton's ascites lymphoma development

被引：3

作者：

Abeesh, Prathapan ^{[1
]}

Guruvayoorappan, Chandrasekharan ^{[1
]}

机构：

[1] Reg Canc Ctr, Div Canc Res, Lab Immunopharmacol & Expt Therapeut, Med Coll Campus, Thiruvananthapuram 695011, Kerala, India

来源：

3 BIOTECH | 2023年 / 13卷 / 06期

关键词：

Umbelliferone; Nanoliposomes; Lymphoma; Tumor mitigation; DRUG-DELIVERY SYSTEM; CELL-CYCLE; ANTICANCER ACTIVITY; ANTITUMOR-ACTIVITY; IN-VITRO; APOPTOSIS; NANOPARTICLES; STABILITY; ARREST; MODEL;

D O I：

10.1007/s13205-023-03615-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Umbelliferone (UB) is a phenylpropanoid-based pharmacologically active agent with promising anti-tumor activities. However, complete elucidation of its therapeutic efficacy remains challenging due to low solubility and bioavailability. The present study aimed to develop a liposomal delivery system for UB to enhance its therapeutic efficacy against Dalton's ascites lymphoma tumor model. Umbelliferone-encapsulated nanoliposomes (nLUB) were prepared using the thin-film hydration method and performed a series of characterizations to confirm successful development. The nLUB showed a particle size of 116 +/- 3.2 nm with a negative surface charge and encapsulation efficiency of 78%. In vitro study results showed that nLUB treatment significantly enhanced cellular uptake and apoptosis induction in lymphoma cells compared to free UB. nLUB treatment significantly stabilized body weight, reduced tumor growth, and improved the serum biochemical and hematological parameters of experimental animals, thereby improving their overall survivability compared to an free UB. Our result indicates that nanoencapsulation enhanced the therapeutic potential of UB, which may find its way to clinical application in the near future.

引用

页数：11

共 42 条

[1] Preparation and characterization of withaferin A loaded pegylated nanoliposomal formulation with high loading efficacy: In vitro and in vivo anti-tumour study [J].