Change of antibiotic resistance in Vibrio spp. during shrimp culture in Shanghai

被引:1
作者
Liu, Jing [1 ]
Wu, Qian [1 ]
Xu, Huan [1 ]
Pan, Yingjie [1 ,2 ,3 ]
Malakar, Pradeep K. [1 ]
Zhao, Yong [1 ,2 ,3 ]
Zhang, Zhaohuan [1 ]
机构
[1] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
[2] Minist Agr & Rural Affairs, Lab Qual & Safety Risk Assessment Aquat Prod Stora, Shanghai 201306, Peoples R China
[3] Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Shanghai 201306, Peoples R China
基金
中国国家自然科学基金; 上海市自然科学基金;
关键词
Shrimp; Aquaculture environment; Vibrio spp; ARGs; Multilocus sequence typing; ANTIMICROBIAL RESISTANCE; BACTERIAL PATHOGENS; UNITED-STATES; PARAHAEMOLYTICUS; PREVALENCE; GENES; AQUACULTURE; SEAFOOD; IDENTIFICATION; DISSEMINATION;
D O I
10.1016/j.aquaculture.2023.740303
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The judicious application of antibiotics in shrimp aquaculture is vital for addressing bacterial infections, yet monitoring bacterial resistance during distinct aquaculture phases remains deficient. This study focused on tracking antibiotic resistance dynamics in Vibrio strains sourced from both shrimp and their breeding settings. Over a standard aquaculture period (day 0, 20, 40, 60, and 80), we isolated eighty-eight Vibrio cholerae and ninety Vibrio parahaemolyticus strains from water, sediment, and shrimp samples. All isolates underwent assessment for antibiotic resistance phenotype and genotype. Both Vibrio species demonstrated resistance to nine antimicrobials. Notably, Vibrio cholerae isolates exhibited resistance rates of up to 46.6% for cephazolin, 30.7% for streptomycin, and 11.4% for ampicillin. Conversely, V. parahaemolyticus isolates displayed pronounced resistance to ampicillin (100%), cephazolin (62.2%), and cefoxitin (36.7%). Moreover, we identified 38 anti-biotic resistance genes (ARGs) across all samples, with a higher prevalence observed in the water. Genetic di-versity and clonal origins of the Vibrio isolates were quantified using Multilocus Sequence Typing (MLST). New Sequence Types (STs) were predominant among V. cholerae (95.4% of 43 isolates) and V. parahaemolyticus (92.9% of 28 isolates). Predominantly, V. parahaemolyticus isolates formed two extensive clonal groups: CC1420 and CC744. As the shrimp cultivation period progressed, both the resistance development rate among isolates and the average ARG count within the same STs of Vibrios steadily increased. This study underscores the potential role of ARGs transmission in the aquaculture environment as a crucial conduit for Vibrio antibiotic resistance development. Insights gained contribute to understanding the dissemination and transmission of antibiotic resistance in aquaculture, aiding in devising strategies to curb antibiotic resistance in aquatic environments.
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页数:12
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共 76 条
  • [41] Antimicrobial Drug Resistance in Fish Pathogens
    Miller, Ron A.
    Harbottle, Heather
    [J]. MICROBIOLOGY SPECTRUM, 2018, 6 (01):
  • [42] Abundance, antimicrobial resistance, and virulence of pathogenic Vibrio strains from molluscan shellfish farms along the Korean coast
    Mok, Jong Soo
    Ryu, Ara
    Kwon, Ji Young
    Park, Kunbawui
    Shim, Kil Bo
    [J]. MARINE POLLUTION BULLETIN, 2019, 149
  • [43] Multi-drug resistant pathogenic bacteria in the gut of young children in Bangladesh
    Monira, Shirajum
    Shabnam, Syeda Antara
    Ali, Sk. Imran
    Sadique, Abdus
    Johura, Fatema-Tuz
    Rahman, Kazi Zillur
    Alam, Nur Haque
    Watanabe, Haruo
    Alam, Munirul
    [J]. GUT PATHOGENS, 2017, 9
  • [44] Prevalence, Virulence Characterization, AMR Pattern and Genetic Relatedness of Vibrio parahaemolyticus Isolates From Retail Seafood of Kerala, India
    Narayanan, Sreejith V.
    Joseph, Toms C.
    Peeralil, Shaheer
    Mothadaka, Mukteswar P.
    Lalitha, Kuttanappilly V.
    [J]. FRONTIERS IN MICROBIOLOGY, 2020, 11
  • [45] Characterization of Vibrio parahaemolyticus isolates from shrimp farms of Southeast coast of India with special reference to Acute Hepatopancreatic Necrosis Disease (AHPND) status
    Navaneeth, K. A.
    Bhuvaneswari, T.
    Rajan, J. J. S.
    Alavandi, S., V
    Vijayan, K. K.
    Otta, S. K.
    [J]. AQUACULTURE, 2020, 518
  • [46] Increasing Rates of Vibriosis in the United States, 1996-2010: Review of Surveillance Data From 2 Systems
    Newton, Anna
    Kendall, Magdalena
    Vugia, Duc J.
    Henao, Olga L.
    Mahon, Barbara E.
    [J]. CLINICAL INFECTIOUS DISEASES, 2012, 54 : S391 - S395
  • [47] Extensive investigation of antimicrobial resistance in Vibrio parahaemolyticus from shellfish and clinical sources, Italy
    Ottaviani, Donatella
    Leoni, Francesca
    Talevi, Giulia
    Masini, Laura
    Santarelli, Sabrina
    Rocchegiani, Elena
    Susini, Francesca
    Montagna, Cosimo
    Monno, Rosa
    D'Annibale, Letizia
    Manso, Esther
    Oliva, Marta
    Pazzani, C.
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 42 (02) : 191 - 193
  • [48] Mobile Genetic Elements Associated with Antimicrobial Resistance
    Partridge, Sally R.
    Kwong, Stephen M.
    Firth, Neville
    Jensen, Slade O.
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 2018, 31 (04)
  • [49] Antibiotic resistance in drinking water systems: Occurrence, removal, and human health risks
    Sanganyado, Edmond
    Gwenzi, Willis
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 669 : 785 - 797
  • [50] Antimicrobial resistance in aquaculture: Current knowledge and alternatives to tackle the problem
    Santos, Lucia
    Ramos, Fernando
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2018, 52 (02) : 135 - 143