A genome-wide association study identifies a transporter for zinc uploading to maize kernels

被引：13

作者：

Chao, Zhen-Fei ^{[1
,2
]}

Chen, Yuan-Yuan ^{[3
]}

Ji, Chen ^{[1
,2
]}

Wang, Ya-Ling ^{[1
]}

Huang, Xing ^{[1
,2
]}

Zhang, Chu-Ying ^{[1
,4
]}

Yang, Jun ^{[5
]}

Song, Tao ^{[1
,2
]}

Wu, Jia-Chen ^{[1
,2
]}

Guo, Liang-Xing ^{[1
,2
]}

Liu, Chu-Bin ^{[1
,2
]}

Han, Mei-Ling ^{[1
]}

Wu, Yong-Rui ^{[1
]}

Yan, Jianbing ^{[3
]}

Chao, Dai-Yin ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

[4] Henan Univ, Sch Life Sci, Kaifeng, Peoples R China

[5] Anhui Agr Univ, Sch Life Sci, Natl Engn Lab Crop Stress Resistance, Hefei, Peoples R China

来源：

EMBO REPORTS | 2023年 / 24卷 / 01期

基金：

中国国家自然科学基金;

关键词：

biofortification; genome-wide association study; maize kernel; YSL2; zinc; GENE-EXPRESSION; IRON UPTAKE; NICOTIANAMINE; BIOFORTIFICATION; ELEMENTS; REVEALS; TRAITS;

D O I：

10.15252/embr.202255542

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Zn content in cereal seeds is an important trait for crop production as well as for human health. However, little is known about how Zn is loaded to plant seeds. Here, through a genome-wide association study (GWAS), we identify the Zn-NA (nicotianamine) transporter gene ZmYSL2 that is responsible for loading Zn to maize kernels. High promoter sequence variation in ZmYSL2 most likely drives the natural variation in Zn concentrations in maize kernels. ZmYSL2 is specifically localized on the plasma membrane facing the maternal tissue of the basal endosperm transfer cell layer (BETL) and functions in loading Zn-NA into the BETL. Overexpression of ZmYSL2 increases the Zn concentration in the kernels by 31.6%, which achieves the goal of Zn biofortification of maize. These findings resolve the mystery underlying the loading of Zn into plant seeds, providing an efficient strategy for breeding or engineering maize varieties with enriched Zn nutrition.

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