Protocol for determining protein dynamics using FT-IR spectroscopy

被引：2

作者：

Shen, Hao ^{[1
,2
]}

Fu, Cuiping ^{[3
]}

Zhang, Junting ^{[3
]}

Feng, Bin ^{[1
,2
]}

Yu, Shaoning ^{[1
,2
]}

机构：

[1] Ningbo Univ, Inst Mass Spectrometry, Sch Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

[3] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

HYDROGEN-DEUTERIUM EXCHANGE; CAP-DNA COMPLEX; MASS-SPECTROMETRY; CRYSTAL-STRUCTURE; RECEPTOR PROTEIN; CAMP; KINASE; ACTIVATION; TRANSITION; RESOLUTION;

D O I：

10.1016/j.xpro.2023.102587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In biological systems, protein function depends on spatial and temporal changes known as protein dynamics, which can be probed by amide hydrogen/deuterium (H/D) exchange. Here, we present a protocol for determining protein dynamics by Fourier-transform infrared (FT-IR) spectroscopy. We describe steps for pro-tein sample preparation and FT-IR spectra collection. We then detail procedures for spectra analysis. Applications include the effects of protein mutation or pro-tein and metal ion or ligand interactions on the protein H/D exchange rate. For complete details on the use and execution of this protocol, please refer to Yu et al. (2013).1

引用

页数：23

共 50 条

[1] SURFACE ANALYSIS USING FT-IR SPECTROSCOPY
ISHIDA, H
KOENIG, JL
AMERICAN LABORATORY, 1978, 10 (03) : 33 - &
[2] SURFACE ANALYSIS USING FT-IR SPECTROSCOPY
ISHIDA, H
KOENIG, JL
INTERNATIONAL LABORATORY, 1978, (MAY-): : 49 - &
[3] FT-IR and NIR spectroscopy
不详
NACHRICHTEN AUS CHEMIE TECHNIK UND LABORATORIUM, 1998, 46 (04): : M1 - +
[4] FT-IR SPECTROSCOPY IN CATALYSIS
MINK, J
ACTA PHYSICA HUNGARICA, 1987, 61 (01) : 71 - 74
[5] APPLICATIONS OF FT-IR SPECTROSCOPY
MOZAYENI, F
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (09) : 1420 - 1426
[6] CHARACTERIZATION OF SEMICONDUCTOR SILICON USING FT-IR SPECTROSCOPY
KRISHNAN, K
MUNDHE, RB
PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS, 1984, 452 : 71 - 78
[7] Assessment of timber biodeterioration using FT-IR spectroscopy
Montaruli, N. E.
de Kuilen, J. W. G. van
Gard, W. F.
Conti, E.
Weersink, R. G. J.
PROTECTION OF HISTORICAL BUILDINGS - PROHITECH 09, VOL 1 AND 2, 2009, : 875 - +
[8] FT-IR spectroscopy inside a glovebox
2007, Advanstar Communications, One Park Avenue, New York, NY 10016, United States (22):
[9] Analysis of diamonds by FT-IR Spectroscopy
Lowry, Stephen
SPECTROSCOPY, 2007, 22 (09) : 38 - 40
[10] MICROBIOLOGICAL CHARACTERIZATIONS BY FT-IR SPECTROSCOPY
NAUMANN, D
HELM, D
LABISCHINSKI, H
NATURE, 1991, 351 (6321) : 81 - 82

← 1 2 3 4 5 →