SINGLE-CELLULAR DYNAMIC MECHANICAL ANALYSIS OF LIVE 3D ORGANOIDS UNDER LIGHT-SHEET FLUORESCENCE MICROSCOPY

被引：0

作者：

Kidambi, Venkatanathan ^{[1
]}

Tomizawa, Yuji ^{[1
]}

Surti, Manav ^{[1
]}

Modarelli, Mitchell ^{[1
]}

Hoshino, Kazunori ^{[1
]}

机构：

[1] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06269 USA

来源：

2024 IEEE 37TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2024年

基金：

美国国家科学基金会;

关键词：

Light sheet microscopy; dynamic mechanical analysis; organoids; mechanical characterization; MECHANOBIOLOGY;

D O I：

10.1109/MEMS58180.2024.10439442

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We have developed a novel microscopic analysis system that combines the functions of light-sheet fluorescence microscopy (LSM) and dynamic mechanical analysis (DMA). We have integrated the three uniquely designed components of (i) a MEMS dynamic compression device with a mu-force sensor, (ii) a high-speed 3D light-sheet scanner and an imager, and (iii) a customprogrammed image-based 3D modeling algorithm. Here, we demonstrate spatially-resolved mechanical characterization of viscoelastic materials under high-resolution 3D fluorescence microscopy for the first time.

引用

页码：330 / 333

页数：4

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