β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs

被引：11
作者：
Li, Leyi [1 ]
Han, Qinghe [2 ]
Chen, Yurong [3 ]
Zhang, Meng [3 ]
Wang, Luyao [3 ]
An, Xinglan [3 ]
Zhang, Sheng [3 ]
Zhai, Yanhui [3 ]
Dai, Xiangpeng [3 ]
Tang, Bo [1 ]
Li, Ziyi [3 ]
Xie, Guanghong [1 ]
机构：
[1] Jilin Univ, Coll Vet Med, Key Lab Zoonosis Res, Minist Educ, Changchun 130021, Jilin, Peoples R China
[2] Second Hosp Jilin Univ, Dept Radiol, Changchun 130041, Peoples R China
[3] First Hosp Jilin Univ, Minist Educ, Key Lab Organ Regenerat & Transplantat, Changchun 130021, Jilin, Peoples R China
来源：
PLOS ONE | 2023年 / 18卷 / 10期
关键词：
MOUSE OOCYTES; OXIDATIVE STRESS; GENE-EXPRESSION; NAD(+); CALCIUM;
D O I：
10.1371/journal.pone.0291640
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Oocyte senescence alters the shape and function, thereby weakening the fertilization potential. Nicotinamide mononucleotide (NMN) reverses age-related dysfunctions in various organs. Studies had shown long-term administration of NMN reduced the physiological decline associated in aged mice and reversed the aging of the ovaries. However, the protective effect of NMN on aged porcine oocytes is still unclear. In this study, we investigated the effects of NMN on aging porcine oocytes and subsequent embryonic development. We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro. NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat. The mitochondrial membrane potential of aged oocytes treated with NMN was increased compared with that of untreated oocytes. In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased. Furthermore, NMN supplementation enhanced the subsequent development ability of senescent oocytes during in vitro aging. Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased. Taken together, these results suggest that NMN is beneficial for delaying the aging process in porcine oocytes.
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页数：13
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