Fission Yeast TORC1 Promotes Cell Proliferation through Sfp1, a Transcription Factor Involved in Ribosome Biogenesis

被引：3

作者：

Tai, Yen Teng ^{[1
,7
]}

Fukuda, Tomoyuki ^{[2
]}

Morozumi, Yuichi ^{[1
]}

Hirai, Hayato ^{[3
]}

Oda, Arisa H. ^{[3
]}

Kamada, Yoshiaki ^{[4
,5
]}

Akikusa, Yutaka ^{[1
]}

Kanki, Tomotake ^{[2
]}

Ohta, Kunihiro ^{[3
]}

Shiozaki, Kazuhiro ^{[1
,6
]}

机构：

[1] Nara Inst Sci & Technol, Div Biol Sci, Nara, Japan

[2] Niigata Univ, Dept Cellular Physiol, Grad Sch Med & Dent Sci, Niigata, Japan

[3] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo, Japan

[4] Natl Inst Basic Biol, Okazaki, Aichi, Japan

[5] Grad Univ Adv Studies SOKENDAI, Hayama, Kanagawa, Japan

[6] Univ Calif Davis, Dept Microbiol & Mol Genet, Davis, CA USA

[7] Penn State Univ, Coll Med, Hershey, PA 17033 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2023年 / 43卷 / 12期

关键词：

fission yeast; rapamycin; ribosome; Sfp1; TORC1; transcription factor; NITROGEN STARVATION; SEXUAL DEVELOPMENT; RAG GTPASES; MOLECULAR-MECHANISMS; GAP ACTIVITY; GROWTH; GENE; COMPLEX; REGULATOR; MTORC1;

D O I：

10.1080/10985549.2023.2282349

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Target of rapamycin complex 1 (TORC1) is activated in response to nutrient availability and growth factors, promoting cellular anabolism and proliferation. To explore the mechanism of TORC1-mediated proliferation control, we performed a genetic screen in fission yeast and identified Sfp1, a zinc-finger transcription factor, as a multicopy suppressor of temperature-sensitive TORC1 mutants. Our observations suggest that TORC1 phosphorylates Sfp1 and protects Sfp1 from proteasomal degradation. Transcription analysis revealed that Sfp1 positively regulates genes involved in ribosome production together with two additional transcription factors, Ifh1/Crf1 and Fhl1. Ifh1 physically interacts with Fhl1, and the nuclear localization of Ifh1 is regulated in response to nutrient levels in a manner dependent on TORC1 and Sfp1. Taken together, our data suggest that the transcriptional regulation of the genes involved in ribosome biosynthesis by Sfp1, Ifh1, and Fhl1 is one of the key pathways through which nutrient-activated TORC1 promotes cell proliferation.

引用

页码：675 / 692

页数：18

共 80 条

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