MIRNA-200B LEVEL IN PERIPHERAL BLOOD PREDICTS RENAL INTERSTITIAL INJURY IN PATIENTS WITH DIABETIC NEPHROPATHY

Background: To uncover the diagnostic potential of periph-eral blood microRNA-200b (miRNA-200b) in renal intersti-tial injury in diabetic nephropathy (DN) patients.Methods: A total of 50 diabetes subjects, 50 mild DN sub-jects, 50 moderate-severe DN subjects and 50 healthy sub-jects were included. Peripheral blood level of miRNA-200b in every subject was detected by reverse transcriptase-poly-merase chain reaction (RT-PCR). Serum levels of renal function indicators were determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, relative levels of fibrosis damage indicators were examined by chemilumi-nescent immunoassay. Diagnostic potentials of miRNA-200b in diabetes, mild DN and moderate-severe DN were assessed by depicting receiver operating characteristic (ROC) curves.Results: Peripheral blood level of miRNA-200b was higher in DN subjects than diabetes subjects without vascular complications, especially moderate-severe DN patients. Peripheral blood level of miRNA-200b in DN subjects was negatively correlated to relative levels of serum creatinine, urinary nitrogen, cystatin, TGF-B, CIV and PCIII. ROC curves demonstrated diagnostic potentials of miRNA-200b in mild and moderate-severe DN.Conclusions: Peripheral blood level of miRNA-200b is closely linked to the degree of renal interstitial injury in DN patients. MiRNA-200b may be a vital indicator in predict-ing the development of DN.