Mycelium-based biomaterials as smart devices for skin wound healing

被引：12

作者：

Ruggeri, Marco ^{[1
]}

Miele, Dalila ^{[1
]}

Contardi, Marco ^{[2
]}

Vigani, Barbara ^{[1
]}

Boselli, Cinzia ^{[1
]}

Cornaglia, Antonia Icaro ^{[3
]}

Rossi, Silvia ^{[1
]}

Suarato, Giulia ^{[2
,4
]}

Athanassiou, Athanassia ^{[2
]}

Sandri, Giuseppina ^{[1
]}

机构：

[1] Univ Pavia, Dept Drug Sci, Pavia, Italy

[2] Ist Italiano Tecnol, Smart Mat, Genoa, Italy

[3] Univ Pavia, Dept Publ Hlth Expt & Forens Med, Pavia, Italy

[4] CNR IEIIT, Ist Elettron Ingn Informaz & Telecomunicazioni, Milan, Italy

来源：

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2023年 / 11卷

关键词：

mycelium; Ganoderma lucidum; Pleurotus ostreatus; biocompatibility; gene expression; murine model; DOWN-REGULATION; STRESS; BCL-2; CELLS;

D O I：

10.3389/fbioe.2023.1225722

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Introduction: Recently, mycelia of Ganoderma lucidum and Pleurotus ostreatus, edible fungi, have been characterized in vitro as self-growing biomaterials for tissue engineering since they are constituted of interconnected fibrous networks resembling the dermal collagen structure.Aim: This work aims to investigate the biopharmaceutical properties of G. lucidum and P. ostreatus mycelia to prove their safety and effectiveness in tissue engineering as dermal substitutes.Methods: The mycelial materials were characterized using a multidisciplinary approach, including physicochemical properties (morphology, thermal behavior, surface charge, and isoelectric point). Moreover, preclinical properties such as gene expression and in vitro wound healing assay have been evaluated using fibroblasts. Finally, these naturally-grown substrates were applied in vivo using a murine burn/excisional wound model.Conclusions: Both G. lucidum and P. ostreatus mycelia are biocompatible and able to safely and effectively enhance tissue repair in vivo in our preclinical model.

引用

页数：10