The MEKK1-MKK1/2-MPK4 cascade phosphorylates and stabilizes STOP1 to confer aluminum resistance in Arabidopsis

被引：34

作者：

Zhou, Fanglin ^{[1
,2
]}

Singh, Somesh ^{[1
]}

Zhang, Jie ^{[1
]}

Fang, Qiu ^{[1
]}

Li, Chongyang ^{[1
]}

Wang, Jiawen ^{[1
]}

Zhao, Chunzhao ^{[1
]}

Wang, Pengcheng ^{[1
]}

Huang, Chao-Feng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci,Natl Key Lab Plan, Shanghai 200032, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

MOLECULAR PLANT | 2023年 / 16卷 / 02期

基金：

中国国家自然科学基金;

关键词：

aluminum resistance; Arabidopsis thaliana; MAPK cascade; MPK4; STOP1; phosphorylation; stability; REGULATES MULTIPLE GENES; ABC TRANSPORTER; FREEZING TOLERANCE; ACTIVATED CITRATE; MPK4; ACTIVATION; PHOSPHOLIPASE-C; CELL-DEATH; PROTEIN; STRESS; MEKK1;

D O I：

10.1016/j.molp.2022.11.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aluminum (Al) toxicity can seriously restrict crop production on acidic soils, which comprise 40% of the world's potentially arable land. The zinc finger transcription factor STOP1 has a conserved and essential function in mediating plant Al resistance. Al stress induces STOP1 accumulation via post-transcriptional regulatory mechanisms. However, the upstream signaling pathway involved in Al-triggered STOP1 accu-mulation remains unclear. Here, we report that the MEKK1-MKK1/2-MPK4 cascade positively regulates STOP1 phosphorylation and stability. Mutations of MEKK1, MKK1/2, or MPK4 lead to decreased STOP1 stability and Al resistance. Al stress induces the kinase activity of MPK4, which interacts with and phos-phorylates STOP1. The phosphorylation of STOP1 reduces its interaction with the F-box protein RAE1 that mediates STOP1 degradation, thereby leading to enhanced STOP1 stability and Al resistance. Taken together, our results suggest that the MEKK1-MKK1/2-MPK4 cascade is important for Al signaling and con-fers Al resistance through phosphorylation-mediated enhancement of STOP1 accumulation in Arabidopsis.

引用

页码：337 / 353

页数：17

共 75 条

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