miRNA-381-3p Functions as a Tumor Suppressor to Inhibit Gastric Cancer by Targeting Fibroblast Growth Factor Receptor-2

被引:4
作者
Gao, Xiang [1 ,2 ,3 ]
Liu, Huiqi [1 ,2 ]
Wu, Qiong [1 ,2 ]
Wang, Rong [1 ,2 ]
Huang, Mingyu [1 ,2 ]
Ma, Qiang [1 ,2 ]
Liu, Yongnian [1 ,2 ]
机构
[1] Qinghai Univ, Key Lab Applicat High Altitude Med, Dept Basic Med Sci, 251 Ningda Rd, Xining 810001, Qinghai, Peoples R China
[2] Qinghai Univ, Key Lab Applicat High Altitude Med, Res Ctr Qinghai Hlth Dev, Xining 810001, Qinghai, Peoples R China
[3] Qinghai Univ, Key Lab Applicat High Altitude Med, Res Ctr High Altitude Med, Xining, Peoples R China
关键词
FGFR2; gastric cancer; metastasis; miR-381-3p; proliferation; CELL-PROLIFERATION; MIR-381-3P; MUTATIONS; MIGRATION; INVASION; FGFR2; PROGRESSION; PATHWAY;
D O I
10.1089/cbr.2021.0357
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objectives: MicroRNAs possess essential effects on gastric cancer (GC), whereas the underlying mechanisms have not been fully uncovered. The present work focused on investigating the role of miR-381-3p in GC cellular processes and the possible mechanisms.Methods: miR-381-3p levels within GC tissues and cells were measured through quantitative real-time polymerase chain reaction (qRT-PCR). This study measured cell proliferation, apoptosis, and metastasis through EdU, colony formation, flow cytometry, and Transwell assays separately. TargetScan was adopted to predict the miR-381-3p targets, whereas luciferase reporter assay was adopted for confirmation.Results: miR-381-3p levels were decreased, whereas fibroblast growth factor receptor-2 (FGFR2) expression was increased in GC. miR-381-3p upregulation inhibited proliferation, migration, and invasion and it promoted the apoptosis of GC cells. Further, FGFR2 overexpression partly reversed the miR-381-3p-mediated impacts on GC cellular processes.Conclusions: This study provides an experimental basis, suggesting the potential of using miR-381-3p as the novel marker for GC. Clinical Trial Registration number: 2020-05.
引用
收藏
页码:396 / 404
页数:9
相关论文
共 43 条
  • [21] MiR-381-3p suppresses biological characteristics of cancer in head-neck squamous cell carcinoma cells by targeting nuclear autoantigenic sperm protein (NASP)
    Kong, Fanyong
    Li, Lianhe
    Wang, Chaoshan
    Zhang, Qiang
    He, Shizhi
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2020, 84 (04) : 703 - 713
  • [22] Kong W, 2018, EUR REV MED PHARMACO, V22, P3804, DOI 10.26355/eurrev_201806_15264
  • [23] Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer
    Li, Jiangfeng
    Ying, Yufan
    Xie, Haiyun
    Jin, Ke
    Yan, Huaqing
    Wang, Song
    Xu, Mingjie
    Xu, Xin
    Wang, Xiao
    Yang, Kai
    Zheng, Xiangyi
    Xie, Liping
    [J]. FASEB JOURNAL, 2019, 33 (01) : 1374 - 1388
  • [24] Anticancer molecules targeting fibroblast growth factor receptors
    Liang, Guang
    Liu, Zhiguo
    Wu, Jianzhang
    Cai, Yuepiao
    Li, Xiaokun
    [J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 2012, 33 (10) : 531 - 541
  • [25] LncRNA FLVCR1-AS1 promotes proliferation, migration and activates Wnt/β-catenin pathway through miR-381-3p/CTNNB1 axis in breast cancer
    Pan, Zhiyu
    Ding, Junbin
    Yang, Zhen
    Li, Huaqing
    Ding, Hongjian
    Chen, Qian
    [J]. CANCER CELL INTERNATIONAL, 2020, 20 (01)
  • [26] Targeting FGFR pathway in breast cancer
    Perez-Garcia, J.
    Munoz-Couselo, E.
    Soberino, J.
    Racca, F.
    Cortes, J.
    [J]. BREAST, 2018, 37 : 126 - 133
  • [27] Frequent activating FGFR2 mutations in endometrial carcinomas parallel germline mutations associated with craniosynostosis and skeletal dysplasia syndromes
    Pollock, P. M.
    Gartside, M. G.
    Dejeza, L. C.
    Powell, M. A.
    Mallon, M. A.
    Davies, H.
    Mohammadi, M.
    Futreal, P. A.
    Stratton, M. R.
    Trent, J. M.
    Goodfellow, P. J.
    [J]. ONCOGENE, 2007, 26 (50) : 7158 - 7162
  • [28] Intestinal Microbiota Is Altered in Patients with Gastric Cancer from Shanxi Province, China
    Qi, Yu-feng
    Sun, Jun-ning
    Ren, Lai-feng
    Cao, Xue-ling
    Dong, Jian-hong
    Tao, Kai
    Guan, Xue-mei
    Cui, Ya-ni
    Su, Wen
    [J]. DIGESTIVE DISEASES AND SCIENCES, 2019, 64 (05) : 1193 - 1203
  • [29] Emerging Role of miRNAs in the Drug Resistance of Gastric Cancer
    Riquelme, Ismael
    Letelier, Pablo
    Riffo-Campos, Angela L.
    Brebi, Priscilla
    Carlos Roa, Juan
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (03):
  • [30] Gastric Cancer: Recent Molecular Classification Advances, Racial Disparity, and Management Implications
    Sanjeevaiah, Aravind
    Cheedella, Naga
    Hester, Caitlin
    Porembka, Matthew R.
    [J]. JOURNAL OF ONCOLOGY PRACTICE, 2018, 14 (04) : 217 - +