Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens

被引：3

作者：

Liu, Dan-Rui ^{[1
]}

Yan, Qing-Xiang ^{[2
]}

Zou, Zheng-Biao ^{[2
]}

Xie, Chun-Lan ^{[2
]}

Yang, Xian-Wen ^{[2
]}

Jia, Ai-Qun ^{[3
]}

机构：

[1] Hainan Univ, Sch Pharmaceut Sci, Key Lab Trop Biol Resources, Minist Educ, Haikou 570228, Peoples R China

[2] Third Inst Oceanog, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China

[3] Hainan Med Univ, Hainan Gen Hosp, Cent Lab, Hainan Affiliated Hosp, Haikou 570311, Peoples R China

来源：

BIOFILM | 2023年 / 6卷

基金：

中国国家自然科学基金;

关键词：

Cladosporium sphaerospermum; Deep-sea-derived fungus; Biofilm; Serratia marcescens; Quorum sensing; BIOFILM FORMATION; METABOLISM;

D O I：

10.1016/j.bioflm.2023.100146

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Serratia marcescens is now becoming a propensity for its highly antimicrobial-resistant clinical infections. Currently, it provides a novel strategy to prevent and control microbial infection by regulating S. marcescens quorum sensing (QS). Deep-sea-derived fungi are rich in QS bioactive constituents. In this work, the extracts from Cladosporium sphaerospermum SCSGAF0054 showed potent QS-related virulence factors and biofilm-inhibiting activities against S. marcescens NJ01. The swimming motility and multiple virulence factors such as prodigiosin, exopolysaccharide (EPS), lipase, protease and hemolysin were moderately inhibited by the extracts at varied concentrations. The confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) images revealed that C. sphaerospermum extracts moderately arrested biofilm formation and cell viability. Further, realtime quantitative PCR (RT-qPCR) analysis revealed that expressions of genes associated with virulence factors, flhD, fimA, fimC, bsmA, bsmB, pigA, pigC, and shlA, were significantly down-regulated compared with control. In addition, the extracts combined with imipenem inhibited the QS system of S. marcescens NJ01, disrupted its preformed biofilm, released the intra-biofilm bacteria and killed the bacteria gradually. Therefore, the extracts combined with imipenem can partially restore bacterial drug sensitivity. These results suggest that the extracts from SCSGAF0054 effectively interfere with the QS system to treat S. marcescens infection alone or combining with classical antimicrobial drugs.

引用

页数：11