Facile one-step synthesis of gold nanoparticles using Viscum album and evaluation of their antibacterial potential

被引:4
作者
Ishaq, Muhammad [3 ]
Numan, Muhammad [3 ]
Zeb, Umar [1 ,4 ,5 ]
Cui, Fengjie [5 ]
Shad, Salma [6 ]
Hayat, Syed Adil [3 ]
Azizullah, Azizullah [4 ]
Uddin, Imad [6 ]
Iqbal, Muzaffar [6 ]
Rahim, Fazli [3 ]
Khan, Naeem [7 ]
Attia, Kotb A. [8 ]
Fiaz, Sajid [2 ,9 ]
机构
[1] Univ Haripur, Fac Biol & Biomed Sci, Dept Biol, Haripur 22620, KPK, Pakistan
[2] Univ Haripur, Dept Plant Breeding & Genet, Haripur 22620, Pakistan
[3] Bacha Khan Univ, Dept Bot, Charsadda 24631, KPK, Pakistan
[4] Univ Haripur, Fac Biol & Biomed Sci, Dept Biol, Haripur 22620, KPK, Pakistan
[5] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[6] Univ Haripur, Fac Nat Sci, Dept Chem, Haripur 22620, KPK, Pakistan
[7] Florida Univ, Inst Food & Agr Sci, Dept Agron, Gainesville, FL 32611 USA
[8] King Saud Univ, Coll Sci, Dept Biochem, POB 2455, Riyadh 11451, Saudi Arabia
[9] Univ Haripur, Dept Plant Breeding & Genet, Haripur 22620, Pakistan
关键词
antibacterial activity; antibacterial potential; Au NPs; cytotoxicity; EDX; SEM; UV-Visual spectral analysis; Viscum album Linn; SILVER NANOPARTICLES; GREEN SYNTHESIS; FLOWER EXTRACT; PHYTOSYNTHESIS; MECHANISM; ROOT;
D O I
10.1071/FP22161
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nanostructure gold nanoparticles (Au NPs) are well-known biological active materials, synthesised under different environment-friendly approaches that has gained significant interest in the field of biomedicine. This study investigated a novel, fast, easy, cost-effective and the eco-friendly method to synthesise Au NPs from mediated Viscum album Linn plant extract, where the plant metabolites act as stabilising and reducing agents. The synthesised Au NPs were analysed by UV/Vis spectroscopy that gave strong signals and a sharp absorption peak at 545 nm due to the presence of surface plasmon resonance (SPR) bands. In addition, energy dispersive X-ray spectroscopy (EDX) showed that strong signals of Au NPs appeared at 9.7 and 2.3 keV, as the rays of light passed. X-ray diffraction recognised the crystalline material and provided information on the cell unit that the synthesised Au NPs are face-centreed cubic in structure. The diffraction of X-ray spectra showed intense peaks at 38.44 degrees, 44.7 degrees, 44.9 degrees and 77.8 degrees. The mediated V. album plant extracts and synthesised Au NPs were screened against gram-positive and gram-negative (Enterobacter, Salmonella typhi, Escheria coli and Bacillus subtilis) bacterial strains, confirming their antibacterial potential. Au NPs showed strong antibacterial activity due to its unique steric configuration. Au NPs damaged bacterial cell membrane leading to the leakage of the cytoplasm and death of the cell.
引用
收藏
页码:955 / 964
页数:10
相关论文
共 60 条
[1]  
Abirami Hariharan Abirami Hariharan, 2016, Pharmacognosy Journal, V8, P507, DOI 10.5530/pj.2016.5.17
[2]   Agriculture nanotechnology: Translating research outcome to field applications by influencing environmental sustainability [J].
Acharya, Amitabha ;
Pal, Probir Kumar .
NANOIMPACT, 2020, 19
[3]   Phytosynthesis and Antileishmanial Activity of Gold Nanoparticles by Maytenus Royleanus [J].
Ahmad, Aftab ;
Syed, Fatima ;
Imran, Muhammad ;
Khan, Arif Ullah ;
Tahir, Kamran ;
Khan, Zia Ul Haq ;
Yuan, Qipeng .
JOURNAL OF FOOD BIOCHEMISTRY, 2016, 40 (04) :420-427
[4]   Green synthesis and chemical characterization of gold nanoparticle synthesized using Camellia sinensis leaf aqueous extract for the treatment of acute myeloid leukemia in comparison to daunorubicin in a leukemic mouse model [J].
Ahmeda, Ahmad ;
Zangeneh, Akram ;
Zangeneh, Mohammad Mahdi .
APPLIED ORGANOMETALLIC CHEMISTRY, 2020, 34 (03)
[5]   Revisiting the mechanistic pathways for bacterial mediated synthesis of noble metal nanoparticles [J].
Ali, Jafar ;
Ali, Naeem ;
Wang, Lei ;
Waseem, Hassan ;
Pan, Gang .
JOURNAL OF MICROBIOLOGICAL METHODS, 2019, 159 :18-25
[6]   Gold and silver nanoparticles used for SERS detection of S. aureus and E. coli [J].
Ankamwar, Balaprasad ;
Gharpure, Saee .
NANO EXPRESS, 2020, 1 (01)
[7]   An eco-friendly method of synthesizing gold nanoparticles using an otherwise worthless weed pistia (Pistia stratiotes L.) [J].
Anuradha, J. ;
Abbasi, Tasneem ;
Abbasi, S. A. .
JOURNAL OF ADVANCED RESEARCH, 2015, 6 (05) :711-720
[8]   Thermoresponsive gels containing gold nanoparticles as smart antibacterial and wound healing agents [J].
Arafa, Mona G. ;
El-Kased, Reham F. ;
Elmazar, M. M. .
SCIENTIFIC REPORTS, 2018, 8
[9]   Pressurized water extraction - the fast and efficient method for isolation of bioactive proteins from Viscum album leaves [J].
Burdejova, Lenka ;
Moravcova, Dana ;
Strouhalova, Dana ;
Lunerova, Kamila .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2021, 195
[10]   Medicinal plants: Treasure trove for green synthesis of metallic nanoparticles and their biomedical applications [J].
Chandra, Harish ;
Kumari, Pragati ;
Bontempi, Elza ;
Yadav, Saurabh .
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2020, 24