Sample preparation and culture condition effects on MALDI-TOF MS identification of bacteria: A review

被引：48
作者：
Topic Popovic, Natalija [1 ,2 ]
Kazazic, Snjezana P. [3 ]
Bojanic, Krunoslav [1 ,2 ]
Strunjak-Perovic, Ivancica [1 ,2 ]
Coz-Rakovac, Rozelindra [1 ,2 ]
机构：
[1] Rudjer Boskovic Inst, Div Mat Chem, Lab Aquaculture Biotechnol, Bijenicka 54, Zagreb 10000, Croatia
[2] Rudjer Boskovic Inst, Ctr Excellence Marine Bioprospecting BioProCro, Zagreb, Croatia
[3] Rudjer Boskovic Inst, Lab Mass Spectrometry & Funct Prote, Zagreb, Croatia
来源：
MASS SPECTROMETRY REVIEWS | 2023年 / 42卷 / 05期
关键词：
bacteria; culture conditions; diagnostics; MALDI-TOF MS; sample preparation; DESORPTION IONIZATION-TIME; LASER-DESORPTION/IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; GRAM-NEGATIVE BACTERIA; STAPHYLOCOCCUS-AUREUS; ROUTINE IDENTIFICATION; PATHOGENIC BACTERIA; MICROBIAL MIXTURES; SPECTRUM QUALITY; STRAIN LEVEL;
D O I：
10.1002/mas.21739
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environmental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDI-TOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have on MALDI-TOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.
引用
收藏
页码：1589 / 1603
页数：15
相关论文
共 94 条
[1]   Microbiota of semen from stallions in Sweden identified by MALDI-TOF [J].
Al-Kass, Ziyad ;
Eriksson, Erik ;
Bagge, Elisabeth ;
Wallgren, Margareta ;
Morrell, Jane M. .
VETERINARY AND ANIMAL SCIENCE, 2020, 10
[2]   Effects of Solid-Medium Type on Routine Identification of Bacterial Isolates by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].
Anderson, Neil W. ;
Buchan, Blake W. ;
Riebe, Katherine M. ;
Parsons, Lauren N. ;
Gnacinski, Stacy ;
Ledeboer, Nathan A. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (03) :1008-1013
[3]   Ion suppression in mass spectrometry [J].
Annesley, TM .
CLINICAL CHEMISTRY, 2003, 49 (07) :1041-1044
[4]   Monitoring the growth of a bacteria culture by MALDI-MS of whole cells [J].
Arnold, RJ ;
Karty, JA ;
Ellington, AD ;
Reilly, JP .
ANALYTICAL CHEMISTRY, 1999, 71 (10) :1990-1996
[5]   Identification of Mycobacteria from Solid and Liquid Media by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in the Clinical Laboratory [J].
Balada-Llasat, J. M. ;
Kamboj, Kamal ;
Pancholi, Preeti .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (09) :2875-2879
[6]   The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling [J].
Balazova, Tereza ;
Makovcova, Jitka ;
Sedo, Ondrej ;
Slany, Michal ;
Faldyna, Martin ;
Zdrahal, Zbynek .
FEMS MICROBIOLOGY LETTERS, 2014, 353 (01) :77-84
[7]  
Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P432, DOI 10.1002/rcm.1290031207
[8]   ALPHA-CYANO-4-HYDROXYCINNAMIC ACID AS A MATRIX FOR MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY [J].
BEAVIS, RC ;
CHAUDHARY, T ;
CHAIT, BT .
ORGANIC MASS SPECTROMETRY, 1992, 27 (02) :156-158
[9]   A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains [J].
Benagli, Cinzia ;
Demarta, Antonella ;
Caminada, AnnaPaola ;
Ziegler, Dominik ;
Petrini, Orlando ;
Tonolla, Mauro .
PLOS ONE, 2012, 7 (10)
[10]  
Bernardo K, 2002, PROTEOMICS, V2, P747, DOI 10.1002/1615-9861(200206)2:6<747::AID-PROT747>3.0.CO
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