Development of a hybridization chain reaction-powered lab-on-fiber device for ultrafast point-of-care testing of circulating tuor DNA in whole blood

被引：9

作者：

Xu, Jiaxin ^{[1
]}

Han, Xiangzhi ^{[1
]}

Xu, Wenjuan ^{[1
]}

Liu, Jiayuan ^{[1
]}

Zhu, Anna ^{[2
]}

Song, Dan ^{[1
]}

Long, Feng ^{[1
]}

机构：

[1] Renmin Univ China, Sch Environm & Nat Resources, Beijing 100872, Peoples R China

[2] State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China

来源：

TALANTA | 2023年 / 259卷

关键词：

Circulating tumor DNA; Hybridization chain reaction; Lab-on-fiber; Evanescent wave fluorescence; Point-of-care testing; TUMOR DNA; SENSITIVE DETECTION; LIQUID BIOPSY; OPPORTUNITIES; CHALLENGES; CELLS; CTDNA; KRAS;

D O I：

10.1016/j.talanta.2023.124475

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Circulating tumor DNA (ctDNA) demonstrates great promise in the guidance of prognostication, diagnosis, and surveillance of cancers, which highlights the need for rapid and sensitive point-of-care testing (POCT) tech-nologies. Hybridization chain reaction (HCR)-based optical biosensors provide excellent solutions due to their prominent features. However, the requirement of a sophisticated and expensive optical readout device, relatively long detection time, and heating hold back their scalability and clinical applications. Here, an innovative HCR-powered lab-on-fiber device (HCR-LOFD) was developed for rapid on-site detection of ctDNA with high sensi-tivity, specificity, and reproducibility. A LOFD with a compact all-fiber optical structure was constructed for the fluorescence detection of the HCR system. Combining HCR, fluorescence energy resonant transfer, and the evanescent wave fluorescence principle, HCR-LOFD achieved the quantitative detection of KRAS G12D, the 12th amino acid from glycine (Gly) mutated aspartate (Asp) and the most common mutation of KARS, in 5 min at room temperature based on end-point detection mode or real-time fluorescence detection mode. This new assay platform was also successfully applied for the direct detection of KRAS G12D in whole blood with simple dilution. The application of HCR-LOFD not only greatly simplifies the complexity of optical readout devices and improves their scalability but also potentially serves as a sample-to-answer solution for the detection of bio-markers in limited medical resource regions.

引用

页数：8