Electrolysis of Bacteria Based on Microfluidic Technology

被引:3
|
作者
Zhao, Jianqiu [1 ]
Li, Na [1 ]
Zhou, Xinyu [1 ]
Yu, Zihan [1 ]
Lan, Mei [1 ]
Chen, Siyu [1 ]
Miao, Jiajia [1 ]
Li, Yulai [1 ]
Li, Guiying [1 ]
Yang, Fang [1 ]
机构
[1] Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
关键词
cell lysis; E; coli; microfluidic; AC electric field; electroporation; CELL-LYSIS; CHIP; ARRAYS; DEVICE;
D O I
10.3390/mi14010144
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell lysis is a key step for studying the structure and function of proteins in cells and an important intermediate step in drug screening, cancer diagnosis, and genome analysis. The current cell lysis methods still suffer from limitations, such as the need for large instruments, a long and time-consuming process, a large sample volume, chemical reagent contamination, and their unsuitability for the small amount of bacteria lysis required for point-of-care testing (POCT) devices. Therefore, a fast, chemical-free, portable, and non-invasive device needs to be developed. In the present study, we designed an integrated microfluidic chip to achieve E. coli lysis by applying an alternating current (AC) electric field and investigated the effects of voltage, frequency, and flow rate on the lysis. The results showed that the lysis efficiency of the bacteria was increased with a higher voltage, lower frequency, and lower flow rate. When the voltage was at 10 Vp-p, the lysis efficiency was close to 100%. The study provided a simple, rapid, reagent-free, and high-efficiency cleavage method for biology and biomedical applications involving bacteria lysis.
引用
收藏
页数:7
相关论文
共 50 条
  • [41] Single-step design of hydrogel-based microfluidic assays for rapid diagnostics
    Puchberger-Enengl, Dietmar
    Krutzler, Christian
    Keplinger, Franz
    Vellekoop, Michael J.
    LAB ON A CHIP, 2014, 14 (02) : 378 - 383
  • [42] Preparation of fish collagen and vancomycin microspheres based on microfluidic technology and its application in osteomyelitis
    Hu, Xiaowu
    Tang, Jinshan
    Yu, Huaixi
    Yang, Hanshi
    Lu, Xiaoqing
    Zheng, Donghui
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2023, 11
  • [43] Folate Receptor-Targeted Albumin Nanoparticles Based on Microfluidic Technology to Deliver Cabazitaxel
    Meng, Fanchao
    Sun, Yating
    Lee, Robert J.
    Wang, Guiyuan
    Zheng, Xiaolong
    Zhang, Huan
    Fu, Yige
    Yan, Guojun
    Wang, Yifan
    Deng, Weiye
    Parks, Emily
    Kim, Betty Y. S.
    Yang, Zhaogang
    Jiang, Wen
    Teng, Lesheng
    CANCERS, 2019, 11 (10)
  • [44] Terahertz microfluidic biosensor based on metamaterial absorber
    Liu, Jianjun
    Fan, Lanlan
    Shen, Xuehua
    OPTOELECTRONICS AND ADVANCED MATERIALS-RAPID COMMUNICATIONS, 2023, 17 (1-2): : 1 - 7
  • [45] The Latest Advances in Microfluidic DLD Cell Sorting Technology: The Optimization of Channel Design
    Fan, Dan
    Liu, Yi
    Liu, Yaling
    BIOSENSORS-BASEL, 2025, 15 (02):
  • [46] Crystal nucleation in a droplet based microfluidic crystallizer
    Teychene, S.
    Biscans, B.
    CHEMICAL ENGINEERING SCIENCE, 2012, 77 : 242 - 248
  • [47] Nanofabrication enabled lab -on -a -chip technology for the manipulation and detection of bacteria
    Li, Lei
    Wang, Cheng
    Nie, Yong
    Yao, Bo
    Hu, Huan
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2020, 127 (127)
  • [48] Microfluidic technology applied to protein sizing and quantitation
    Barthmaier, P
    NANOTECH 2003, VOL 1, 2003, : 67 - 69
  • [49] Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics
    Berlanda, Simon F.
    Breitfeld, Maximilian
    Dietsche, Claudius L.
    Dittrich, Petra S.
    ANALYTICAL CHEMISTRY, 2021, 93 (01) : 311 - 331
  • [50] Application of microfluidic technology in cancer research and therapy
    Azadi, Shohreh
    Es, Hamidreza Aboulkheyr
    Kulasinghe, Arutha
    Bordhan, Pritam
    Warkiani, Majid Ebrahimi
    ADVANCES IN CLINICAL CHEMISTRY, VOL 99, 2020, 99 : 193 - 235