Characteristics of the multiple replicon plasmid IncX1-X1 in multidrug-resistant Escherichia coli from Malayan pangolin (Manis java']javanica)

被引：6

作者：

Ji, Fang ^{[1
]}

Liu, Shelan ^{[2
]}

Wang, Xue ^{[1
]}

Zhao, Jianan ^{[1
]}

Zhu, Jiayue ^{[3
]}

Yang, Jianchun ^{[1
]}

Zhang, Chenglin ^{[4
]}

Jia, Zhongxin ^{[1
]}

Zhao, Ruili ^{[5
]}

Hu, Guocheng ^{[6
]}

Wang, Jing ^{[7
]}

Qin, Jianhua ^{[8
]}

Li, Gang ^{[1
]}

Wu, Bin ^{[1
]}

Wang, Chengmin ^{[1
]}

机构：

[1] Guangdong Acad Sci, Guangdong Key Lab Anim Conservat & Resource Utili, Guangdong Publ Lab Wild Anim Conservat & Utilizat, Inst Zool, Guangzhou 510260, Guangdong, Peoples R China

[2] Zhejiang Ctr Dis Control & Prevent, Dept Infect Dis, Hangzhou, Zhejiang, Peoples R China

[3] East China Univ Sci & Technol, Sch Bioengn, Shanghai, Peoples R China

[4] Beijing Zoo, Beijing Key Lab Capt Wildlife Technol, Beijing, Peoples R China

[5] Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin, Peoples R China

[6] Minist Ecol & Environm, South China Inst Environm Sci, Guangzhou, Peoples R China

[7] Hangzhou Ctr Dis Control & Prevent, Dept Infect Dis, Hangzhou, Peoples R China

[8] Agr Univ Hebei, Coll Vet Med, Baoding, Peoples R China

来源：

INTEGRATIVE ZOOLOGY | 2023年 / 18卷 / 02期

关键词：

ARG; Manis [!text type='java']java[!/text]nica; multiple-replicon plasmid IncX1-X1; plasmid IncX1; COMPLETE SEQUENCES; GENE; DIVERSITY; INTEGRONS; MCR-1; ZOO;

D O I：

10.1111/1749-4877.12637

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Potential zoonotic pathogens may be transmitted from wildlife to humans through the illegal wild meat trade, which has become a pressing issue. However, research on the antimicrobial resistance genes (ARGs) of Malayan pangolin (Manis javanica) intestinal bacteria is limited. Here, multidrug-resistant Escherichia coli M172-1 (ST354) isolated from Malayan pangolin feces in 2019 was found to be resistant to 13 antibiotics. BGWAS analysis revealed 4 plasmids, namely, pM172-1.1, pM172-1.2, pM172-1.3, and pM172-1.4, in the isolate. The pM172-1.2, pM172-1.3, and pM172-1.4 plasmids carried ARGs, namely, IncHI2-HI2A, IncX1-X1, and IncX1, respectively. pM172-1.3 and pM172-1.4 contained intact IntI1 integrons (Is26/IntI1/arr2/cmlA5/bla(OXA-10)/ant(3 '')-IIA/dfrA14/Is26). Notably, pM172-1.3 resulted from the fusion of 2 pM172-1.4 copies and carried many more ARGs. In addition to pM172-1.3 from the same host, other drug-resistant bacteria (E. coli M159-1 (ST48), E. coli S171-1 (ST206), and Klebsiella pneumoniae S174-1 (ST2354)) in the same Malayan pangolin fecal samples also carried 3 plasmids with 100% gene coverage of pM172-1.4 and 99.98% identity. Therefore, ARGs in IncX1 might spread in the intestinal flora of Malayan pangolin and between species via the illegal food chain, posing a potential threat to public health and safety.

引用

页码：289 / 298

页数：10

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