Propolis anti-inflammatory effects on MAGE-1 and retinoic acid-treated dendritic cells and on Th1 and T regulatory cells

被引：0

作者：

Santiago, Karina Basso ^{[1
]}

Conti, Bruno Jose ^{[1
]}

Cardoso, Eliza de Oliveira ^{[1
]}

Conte, Fernanda Lopes ^{[1
]}

Tasca, Karen Ingrid ^{[1
]}

Romagnoli, Graziela Gorete ^{[1
]}

Golim, Marjorie de Assis ^{[2
]}

Cruz, Maria Tereza ^{[3
]}

Sforcin, Jose Mauricio ^{[1
]}

机构：

[1] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP, Brazil

[2] Sao Paulo State Univ UNESP, Sch Med FMB, Botucatu, SP, Brazil

[3] Univ Coimbra, Fac Pharm, Ctr Neurosci & Cellular Biol, Coimbra, Portugal

来源：

JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES | 2023年 / 29卷

基金：

巴西圣保罗研究基金会;

关键词：

Dendritic cells; T CD4+cells; MAGE-1; Retinoic acid; Propolis; Immunomodulation; P-COUMARIC ACID; CYTOKINE PRODUCTION; IMMUNOMODULATORY ACTIVITY; BIOLOGICAL-PROPERTIES; CHEMICAL-COMPOSITION; ANTIGEN-PRESENTATION; GREEN PROPOLIS; EXPRESSION; ACTIVATION; IMMUNITY;

D O I：

10.1590/1678-9199-JVATITD-2022-0044

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Background: Propolis exhibits huge potential in the pharmaceutical industry. In the present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) and on T lymphocytes to observe a possible differential activation of T lymphocytes, driving preferentially to Th1 or Treg cells.Methods: Cell viability, lymphocyte proliferation, gene expression (T-bet and FoxP3), and cytokine production by DCs (TNF-alpha, IL-10, IL-6 and IL-1 beta) and lymphocytes (IFN-gamma and TGF-beta) were analyzed.Results: MAGE-1 and RA alone or in combination with propolis inhibited TNF-alpha production and induced a higher lymphoproliferation compared to control, while MAGE-1 + propolis induced IL-6 production. Propolis in combination with RA induced FoxP3 expression. MAGE-1 induced IFN-gamma production while propolis inhibited it, returning to basal levels. RA inhibited TGF-beta production, what was counteracted by propolis.Conclusion: Propolis affected immunological parameters inhibiting pro-inflammatory cytokines and favoring the regulatory profile, opening perspectives for the control of inflammatory conditions.

引用

页数：12

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