Sensitive lateral flow immunoassay strips based on Fe3+-chelated polydopamine nanospheres for the detection of kanamycin

被引：30

作者：

Liu, Tingting ^{[1
]}

Lai, Xiaocui ^{[1
]}

Guo, Ping ^{[2
]}

Zhang, Wei ^{[2
]}

Zhang, Gan ^{[1
]}

Wu, Mengyun ^{[1
]}

Xue, Guangjian ^{[1
]}

Fang, Xuechen ^{[1
]}

Peng, Juan ^{[1
]}

Lai, Weihua ^{[1
,3
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

[2] Jiangxi Gen Inst Testing & Certificat, Nanchang 330029, Peoples R China

[3] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 411卷

关键词：

KAN monoclonal antibody; Fe 3+-chelated polydopamine nanospheres; Lateral flow immunoassay; Pork; Milk; Honey; IMMUNOCHROMATOGRAPHIC ASSAY; QUANTITATIVE DETECTION; ELISA;

D O I：

10.1016/j.foodchem.2023.135511

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

As kanamycin (KAN) residue in animal products is harmful to consumers, a rapid and sensitive method for KAN detection needs to be established. KAN monoclonal antibody (KAN-mAb, 1D11) with the half maximal inhibitory concentration of 1.16 ng/mL was prepared in this study. A one-pot method was used to synthesize Fe3+-chelated polydopamine nanospheres (Fe@PDANs) with excellent characteristics of strong light absorption. The novel label of Fe@PDANs and KAN-mAb was used to develop a lateral flow immunoassay (LFIA) for the sensitive detection of KAN. The limit of detection of the Fe@PDANs-based LFIA (Fe@PDANs-LFIA) for KAN was 0.0191 ng/mL, which was 2.75 times lower than PDANs-based LFIA. Furthermore, the Fe@PDANs-LFIA was successfully applied to detect KAN in pork, milk, and honey samples, with recoveries ranging from 93.75% to 113.80% (coefficient of variation < 10%). Therefore, Fe@PDANs have potential for the detection of analytes in LFIA.

引用

页数：9

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