Mechanistic Studies of Arene-Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs

被引：9

作者：

Hikisz, Pawel ^{[1
]}

Namiecinska, Ewelina ^{[2
]}

Paneth, Piotr ^{[3
]}

Budzisz, Elzbieta ^{[2
]}

机构：

[1] Univ Lodz, Fac Biol & Environm Protect, Dept Oncobiol & Epigenet, Pomorska 141-143, PL-90236 Lodz, Poland

[2] Med Univ Lodz, Dept Chem Cosmet Raw Mat, Muszynskiego 1, PL-90151 Lodz, Poland

[3] Tech Univ Lodz, Inst Appl Radiat Chem, Dept Chem, Zeromskiego 116, PL-90924 Lodz, Poland

来源：

MOLECULES | 2023年 / 28卷 / 09期

关键词：

anticancer activity; molecular docking; arene-ruthenium(II) complexes; carbothioamidopyrazoles; ARENE RUTHENIUM(II) COMPLEXES; IN-VITRO; ANTIPROLIFERATIVE ACTIVITY; BIOLOGICAL-ACTIVITY; ANTICANCER; DERIVATIVES; LIGANDS; INHIBITION; BEARING; BINDING;

D O I：

10.3390/molecules28093969

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arene-ruthenium(II) complexes with carbothioamidopyrazoles at the C-2 and C-5 positions have been recognized as chemotherapeutic agent alternatives to cisplatin and its oxaliplatin analogs. The aim of this study was to continue research on the biological aspect of arene-ruthenium(II) complexes and their anticancer activity. The present paper includes an additional 12 new tumor cells, analyzed by MTT, and employs a series of extended bioassays to better understand their potential mechanism of antitumor activity. The following tests were conducted: membrane permeability studies, intramolecular reactive oxygen and nitrogen species (ROS/RNS) assays, mitochondrial potential changes, DNA analysis by comet assay using the electrophoresis method, measurement of cleaved PARP protein levels, and determination of apoptotic and necrotic cell fractions by fluorescence microscopy. Additionally, the article presents lipophilicity studies based on RP-TLC and molecular docking studies. We hope that the presented data will prove useful in practical treatment, especially for patients with cancer.

引用

页数：29

共 72 条

[61] Synthesis and molecular structure of arene ruthenium(II) benzhydrazone complexes: impact of substitution at the chelating ligand and arene moiety on antiproliferative activity [J].

Subarkhan, Mohamed Kasim Mohamed ;

Ramesh, Rengan ;

Liu, Yu .

NEW JOURNAL OF CHEMISTRY, 2016, 40 (11) :9813-9823

[62] Ruthenium(II) p-cymene complexes of naphthoquinone derivatives as antitumor agents: A structure-activity relationship study [J].

Tabrizi, Leila ;

Chiniforoshan, Hossein .

JOURNAL OF ORGANOMETALLIC CHEMISTRY, 2016, 822 :211-220

[63] The 1,3-diaryltriazenido(p-cymene)ruthenium(II) complexes with a high in vitro anticancer activity [J].

Vajs, Jure ;

Steiner, Ivana ;

Brozovic, Anamaria ;

Pevec, Andrej ;

Ambriovic-Ristov, Andreja ;

Matkovic, Marija ;

Piantanida, Ivo ;

Urankar, Damijana ;

Osmak, Maja ;

Kosmrlj, Janez .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2015, 153 :42-48

[64] NMR evidence for syn-anti interconversion of a trans opened (10R)-dA adduct of benzo[a]pyrene (7S,8R)-diol (9R,10S)-epoxide in a DNA duplex [J].

Volk, DE ;

Rice, JS ;

Luxon, BA ;

Yeh, HJC ;

Liang, C ;

Xie, G ;

Sayer, JM ;

Jerina, DM ;

Gorenstein, DG .

BIOCHEMISTRY, 2000, 39 (46) :14040-14053

[65] In vitro evaluation of cytotoxicity and antimetastatic properties of novel arene ruthenium(II)-tetrazolato compounds on human cancer cell lines [J].

Vyas, Komal M. ;

Sharma, Deepu ;

Magani, Sri Krishna Jayadev ;

Mobin, Shaikh M. ;

Mukhopadhyay, Suman .

APPLIED ORGANOMETALLIC CHEMISTRY, 2021, 35 (05)

[66] Balanced basis sets of split valence, triple zeta valence and quadruple zeta valence quality for H to Rn: Design and assessment of accuracy [J].

Weigend, F ;

Ahlrichs, R .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2005, 7 (18) :3297-3305

[67] Combination of metallomics and proteomics to study the effects of the metallodrug RAPTA-T on human cancer cells [J].

Wolters, Dirk A. ;

Stefanopoulou, Maria ;

Dyson, Paul J. ;

Groessl, Michael .

METALLOMICS, 2012, 4 (11) :1185-1196

[68] Mitochondria-targeted half-sandwich rutheniumII diimine complexes: anticancer and antimetastasis via ROS-mediated signalling [J].

Xu, Zhishan ;

Kong, Deliang ;

He, Xiangdong ;

Guo, Lihua ;

Ge, Xingxing ;

Liu, Xicheng ;

Zhang, Hairong ;

Li, Juanjuan ;

Yang, Yuliang ;

Liu, Zhe .

INORGANIC CHEMISTRY FRONTIERS, 2018, 5 (09) :2100-2105

[69] Imine-N-Heterocyclic Carbenes as Versatile Ligands in Ruthenium(II) p-Cymene Anticancer Complexes: A Structure-Activity Relationship Study [J].

Yang, Yuliang ;

Guo, Lihua ;

Tian, Zhenzhen ;

Liu, Xicheng ;

Gong, Yuteng ;

Zheng, Hongmei ;

Ge, Xingxing ;

Liu, Zhe .

CHEMISTRY-AN ASIAN JOURNAL, 2018, 13 (19) :2923-2933

[70] Role of Metalation in the Topoisomerase Ilα Inhibition and Antiproliferation Activity of a Series of α-Heterocyclic-N4-Substituted Thiosemicarbazones and Their Cu(II) Complexes [J].

Zeglis, Brian M. ;

Divilov, Vadim ;

Lewis, Jason S. .

JOURNAL OF MEDICINAL CHEMISTRY, 2011, 54 (07) :2391-2398

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