OMIP-090: A 20-parameter flow cytometry panel for rapid analysis of cell diversity and homing capacity in human conventional and regulatory T cells

被引:6
|
作者
Stroukov, Wladislaw [1 ]
Mastronicola, Daniela [1 ]
Albany, Caraugh Jane [1 ,2 ]
Catak, Zeynep [1 ]
Lombardi, Giovanna [1 ]
Scotta, Cristiano [1 ]
机构
[1] Kings Coll London, Sch Immunol & Microbiol Sci, Peter Gorer Dept Immunobiol, London, England
[2] Kings Coll London, British Heart Fdn Ctr, Sch Cardiovasc Med & Sci, London, England
基金
英国惠康基金;
关键词
cell subpopulations; cell trafficking; helper T cells; inflammation; regulatory T cells; tissue homing; B-CELL; SUBSETS; DIFFERENTIATION; RECEPTORS; SKIN; TH17; LYMPHOCYTE; EXPRESSION; STABILITY; MIGRATION;
D O I
10.1002/cyto.a.24720
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The panel was developed and optimized for monitoring changes in homing capacity and functional diversity of human CD4(+) conventional and regulatory T cell subsets. The analysis was based on expression of only surface markers in freshly isolated peripheral blood mononuclear cells (PBMCs) to reduce at minimum any alteration due to permeabilization or freezing/thawing procedures. We included markers to assess the distribution of naive and memory populations based on the expression of CD45RA, CCR7, CD25, CD28 and CD95 in both conventional and regulatory T cells. The identification of major functional subsets was performed using CCR4, CCR6, CCR10, CXCR3 and CXCR5. Homing capacity of these subsets to skin, airway tract, gut and inflammatory lesions could finally be assessed with the markers CLA, CCR3, CCR5 and integrin beta 7. The panel was tested on freshly isolated PBMCs from healthy donors and patients with allergic rhinitis or autoimmune disorders.
引用
收藏
页码:362 / 367
页数:6
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