Sublingual prophylactic administration of OVA-loaded MSC-derived exosomes to prevent allergic sensitization

被引：20

作者：

Asadirad, Ali ^{[1
,2
,3
]}

Ghadiri, Ata. A. ^{[1
]}

Amari, Afshin ^{[1
]}

Dehcheshmeh, Mohammad Ghasemi ^{[1
]}

Sadeghi, Mahvash ^{[1
,3
,4
,6
]}

Dehnavi, Sajad ^{[5
]}

机构：

[1] Ahvaz Jundishapur Univ Med Sci, Fac Med, Dept Immunol, Ahvaz, Iran

[2] Ahvaz Jundishapur Univ Med Sci, Petr & Environm Pollutants Res Ctr, Canc, Ahvaz, Iran

[3] Ahvaz Jundishapur Univ Med Sci, Med Basic Sci Res Inst, Cellular & Mol Res Ctr, Ahvaz, Iran

[4] Ahvaz Jundishapur Univ Med Sci, Student Res Comm, Ahvaz, Iran

[5] Mashhad Univ Med Sci, Immunol Res Ctr, Mashhad, Iran

[6] Ahvaz Jundishapur Univ Med Sci, Dept Immunol, Ahvaz, Iran

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2023年 / 120卷

关键词：

Prophylaxis; Sublingual route; Mesenchymal stem cell (MSC); Exosome; Delivery system; IMMUNOTHERAPY; DELIVERY; EFFICACY; RHINITIS;

D O I：

10.1016/j.intimp.2023.110405

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim: This study evaluated the immunomodulatory and delivery potential of adipose tissue-isolated MSC-derived exosomes as a prophylactic regimen through a sublingual route in the ovalbumin (OVA)-induced allergic asthma murine model.Material and methods: Balb/c mice received 10 & mu;g/dose of OVA-enriched MSC-derived exosomes as a prophylactic regimen in six doses during three weeks, and then OVA sensitization was conducted through intraperitoneal and aerosol administration of allergen. The total cells and eosinophils counted in nasal lavage fluid (NALF) and lung tissues were assessed for histopathological analysis. In addition, the secretion of IFN-& gamma;, IL-4, and TGF-& beta; by spleen cells and serum OVA-specific IgE levels were measured via ELISA.Results: Significant reduction in the IgE levels and IL-4 production, along with elevated TGF-& beta; levels, were observed. Also, limited cellular infiltrations and perivascular and peribronchiolar inflammation in the lung tissues and normal total numbers of cells and eosinophils in the NALF were reported.Conclusion: Prophylactic regimen using OVA-enriched MSC-derived exosomes modulated immune responses and inhibited allergic OVA sensitization.

引用

页数：6

共 32 条

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