Corneal Densitometry with Galilei Dual Scheimpflug Analyzer

This study aims to apply the densitometry distribution analysis (DDA) method to study corneal densitometry depending on age and corneal region from Galilei Dual Scheimpflug Analyzer tomography. A total of 83 healthy participants aged 39.02 +/- 18.34 years (range 9-81 years) were screened using a Ziemer Galilei G2. Images were analysed using the DDA, and two parameters, alpha (corneal transparency) and beta (corneal homogeneity), were estimated. A two-way ANOVA analysis was performed to investigate whether alpha and beta are influenced by age, corneal region (four concentric areas were considered), and their interaction. The parameters alpha and beta statistically change with age and corneal region. A statistically significant interaction effect of 13% (alpha) and 11% (beta) exists between age and corneal region. However, the corneal region plays a more significant role than aging in corneal densitometry; 31% (alpha) and 51% (beta) of the variance can be attributed to the corneal region, while 28% (alpha) and 5% (beta) can be attributed solely to aging. Corneal densitometry can be objectively assessed from Galilei G2 images using the DDA method. The corneal region plays a more significant role than aging in corneal densitometry. Consequently, general results on corneal densitometry and aging should be taken cautiously.