Integration of non-targeted multicomponent profiling, targeted characteristic chromatograms and quantitative to accomplish systematic quality evaluation strategy of Huo-Xiang-Zheng-Qi oral liquid

被引：6

作者：

Li, Xuejuan ^{[1
,3
]}

Wu, Mengfan ^{[1
,3
]}

Ding, Hui ^{[1
,3
]}

Li, Wei ^{[1
,3
]}

Yin, Jiaxin ^{[1
,3
]}

Lin, Ruimei ^{[1
,3
]}

Wu, Xinlong ^{[1
,3
]}

Han, Lifeng ^{[2
,3
]}

Yang, Wenzhi ^{[2
,3
]}

Bie, Songtao ^{[1
,2
,3
]}

Li, Fangyi ^{[1
,2
,3
]}

Song, Xinbo ^{[1
,2
,3
]}

Yu, Heshui ^{[1
,2
,3
]}

Dong, Ziliang ^{[4
]}

Li, Zheng ^{[1
,2
,3
]}

机构：

[1] Tianjin Univ Tradit Chinese Med, Coll Pharmaceut Engn Tradit Chinese Med, Tianjin 301617, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, State Key Lab Component based Chinese Med, Tianjin 301617, Peoples R China

[3] Tianjin Univ Tradit Chinese Med, Haihe Lab Modern Chinese Med, Tianjin 301617, Peoples R China

[4] Chongqing Taiji Ind Grp Co Ltd, Chongqing 408000, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2023年 / 236卷

关键词：

Huo-Xiang-Zheng-Qi oral liquid; Characteristic chromatograms; Selective ion monitoring; Authentication; Quality evaluation; COLLISION CROSS-SECTION; HIGH-RESOLUTION; PATENT MEDICINES; MASS; IDENTIFICATION; MARKERS; FINGERPRINT; RADIX;

D O I：

10.1016/j.jpba.2023.115715

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Huo-Xiang-Zheng-Qi oral liquid (HXZQOL) is a well-known traditional Chinese medicine formula for the treatment of gastrointestinal diseases, with the pharmacologic effects of antiinflammatory, immune protection and gastrointestinal motility regulation. More significantly, HXZQOL is recommended for the treatment of COVID-19 patients with gastrointestinal symptoms, and it has been clinically proven to reduce the inflammatory response in patients with COVID-19. However, the effective and overall quality control of HXZQOL is currently limited due to its complex composition, especially the large amount of volatile and non-volatile active components involved. In this study, aimed to fully develop a comprehensive strategy based on non-targeted multicomponent identification, targeted authentication and quantitative analysis for quality evaluation of HXZQOL from different batches. Firstly, the non-targeted high-definition MSE (HDMSE) approach is established based on UHPLC/IMQTOF-MS, utilized for multicomponent comprehensive characterization of HXZQOL. Combined with in house library-driven automated peak annotation and comparison of 47 reference compounds, 195 components were initially identified. In addition, HS-SPME-GC-MS was employed to analyze the volatile organic compounds (VOCs) in HXZQOL, and a total of 61 components were identified by comparison to the NIST database, reference compounds as well as retention indices. Secondly, based on the selective ion monitoring (SIM) of 24 "identity markers" (involving each herbal medicine), characteristic chromatograms (CCs) were established on LC-MS and GC-MS respectively, to authenticate 15 batches of HXZQOL samples. The targeted-SIM CCs showed that all marker compounds in 15 batches of samples could be accurately monitored, which could indicate preparations authenticity. Finally, a parallel reaction monitoring (PRM) method was established and validated to quantify the nine compounds in 15 batches of HXZQOL. Conclusively, this study first reports chemical-material basis, SIM CCs and quality evaluation of HXZQOL, which is of great implication to quality control and ensuring the authenticity of the preparation.

引用

页数：11

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