Protocol Enzyme-free isolation of mesenchymal stem cells from decidua basalis of the human placenta

被引：4

作者：

Gupta, Srishti Dutta ^{[1
]}

Sen, Ankita ^{[1
]}

Priyadarshi, Priyanshu ^{[1
]}

Ta, Malancha ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res Kolkata IISER Kolkata, Dept Biol Sci, Nadia 741246, W Bengal, India

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 03期

关键词：

Cell Biology; Cell Culture; Cell Differentiation; Cell Isolation; Flow Cytometry/Mass Cytometry; Stem Cells;

D O I：

10.1016/j.xpro.2023.102498

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesenchymal stem cells (MSCs), also referred to as "medicinal signaling cells,"have gained prominence as candidates for cell-based therapy and in clinical trials owing to their regenerative and therapeutic properties. Here, we present a protocol for isolating MSCs from the decidua basalis layer of human placenta using an explant culture approach. We describe steps for collecting, disinfecting, and plating placental tissue. We then detail procedures for characterizing the isolated MSCs through flow cytometry and in vitro differentiation.

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页数：20

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